Fig. 2

Departures from Mendelian (1:1) segregation ratios in progeny embryos at 15 marker loci. a Observed proportions of progenies with significant non-Mendelian ratios (k > 0.5, one-tailed exact binomial tests, p < 0.05) at each locus. Blue-shaded bars represent the three supergene-associated loci; the hatched bar represents the proportion of departures based on simultaneous consideration of all three supergene-associated loci. The dotted line represents the mean proportion across the remaining twelve (non-supergene) loci (6.0%). Error bars for the observed departures represent the one-tailed lower 95% confidence limits derived from bootstrap/rarefaction analyses (Additional file 6: Text S2); bars that do not extend below 0.05 signify significant TRD for that locus. Inset―Observed proportions of progenies with k ≥ 0.65 (the threshold above which segregation ratios generally depart significantly from 1:1 with our sample sizes). Loci are arranged in the order: red_ant, C27, C536, i_114, Sol-42f, i_109, cassidy, Sol-49, i_129, i_120, sunrise, Bertha, Gp-9, C294, and i_126; supergene loci are represented by blue-shaded bars. b Boxplots depicting segregation proportions (k) for each segregating progeny at each locus; k values (unpolarized) represent the more common gamete allele in each such progeny in this graph. The boxes depict the interquartile ranges, with black horizontal lines representing the median and red lines the mean for each locus. Individual progenies with significant non-Mendelian ratios (binomial tests, p < 0.05) are indicated by the larger yellow circles. Supergene loci are represented by boxes shaded blue; loci in this graph are arranged from low to high mean k values