TY - JOUR AU - Islam, Md Tarikul AU - Sarker, Suprovath Kumar AU - Talukder, Shezote AU - Bhuyan, Golam Sarower AU - Rahat, Asifuzzaman AU - Islam, Nafisa Nawal AU - Mahmud, Hasan AU - Hossain, Mohammad Amir AU - Muraduzzaman, A. K. M. AU - Rahman, Jakia AU - Qadri, Syeda Kashfi AU - Shahidullah, Mohammod AU - Mannan, Mohammad Abdul AU - Tahura, Sarabon AU - Hussain, Manzoor AU - Saha, Narayan AU - Akhter, Shahida AU - Nahar, Nazmun AU - Begum, Firoza AU - Shirin, Tahmina AU - Akhteruzzaman, Sharif AU - Qadri, Syed Saleheen AU - Qadri, Firdausi AU - Mannoor, Kaiissar PY - 2018 DA - 2018/08/10 TI - High resolution melting curve analysis enables rapid and reliable detection of G6PD variants in heterozygous females JO - BMC Genetics SP - 58 VL - 19 IS - 1 AB - Like glucose-6-phosphate dehydrogenase (G6PD) deficient hemizygous males and homozygous females, heterozygous females could also manifest hemolytic crisis, neonatal hyperbilirubinemia or kernicterus upon exposure to oxidative stress induced by certain foods such as fava beans, drugs or infections. Although hemizygous males and homozygous females are easily detected by conventional G6PD enzyme assay method, the heterozygous state could be missed by the conventional methods as the mosaic population of both normal and deficient RBCs circulates in the blood. Thus the present study aimed to apply high resolution melting (HRM) curve analysis approach to see whether HRM could be used as a supplemental approach to increase the chance of detection of G6PD heterozygosity. SN - 1471-2156 UR - https://doi.org/10.1186/s12863-018-0664-1 DO - 10.1186/s12863-018-0664-1 ID - Islam2018 ER -