Fig. 5

a Partial sequence profiles of amplicon from Hfr-1 with primer pair Hfr-1_F4 and Hfr-1_R4 for 1 R cultivar, CASW02GH00010S, and 1 S cultivar Cham6. The indicated SNP, A/G is located at nt position 883 of Hfr-1 mRNA, GenBank AF483596.1, which results in an amino acid substitution of isoleucine to valine. This SNP is located in the jacalin-like plant lectin domain of the gene. b The endpoint fluorescence in the genotyping of SNP 883 in exon IV of Hfr-1 using the SNP assay, SNP883_Hfr-1 (Table 2). This SNP segregates between the R cultivars with ‘G’ (VIC; green triangles on y-axis), and the S cultivars with either the ‘A’ (FAM; blue triangles on x-axis) or the heterozygotes (red triangles). This SNP results in an amino acid change from isoleucine to valine in R cultivars. The ‘G’ allele is present in 14 of the 39 R cultivars (Table 3). No S cultivar has the ‘G’ allele (Table 3)