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Table 2 The list of primers for high resolution melting curve analysis for detection of beta-globin gene mutations

From: High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Sl No

Primer name

Primer sequence (5′ → 3′)

Primer size

1

P1

ATGGTGCATCTGACTCCTGAG

21

2

R1

CCAATAGGCAGAGAGAGTCAGTG

23

3

P2

CACTGACTCTCTCTGCCTATTGG

23

4

R2

CAGGCCATCACTAAAGGCACC

21

  1. P1R1 = 1st set of primers for HRM; P2R2 = 2nd set of primers for HRM
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BMC Genomic Data

ISSN: 2730-6844

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