Fig. 1

Scheme of targeted locus in wild-type and genome modified animals. a Ideogram of mouse chromosome 6 and detailed organization of the region limited by 103,500,000 and 106,800,000 positions which contains Chl1, Cntn6 and Cntn4 genes. Red arrows indicate positions sites for primers: FWD1.1, FWD1.2, REV1, FWD2.1 or FWD2.2, and REV2. These sites mark the boundaries of the presumable 1137 Mb deletion. b Genotyping of a deletion involving the Cntn6 gene by primers FWD1.1 and REV2. Left and right shoulders of ssODN are shown red and blue, respectively. c Genotyping of duplication involving the Cntn6 gene by primers FWD2.1 and REV1. d Genotyping of inversion involving the Cntn6 gene by two pairs of primers: FWD1.1 or FWD1.2 (FWD1.2 was used only for prepare PCR-product for sequencing) and FWD2.2 for left side, and REV1 and REV2 for right side, respectively. e Exp Del, Exp Dup, Exp Inv-L and Exp Inv-R are expected sequences in new joint sites for deletion, duplication and inversions, respectively, due to correct reparation after Cas9-nuclease digest between 3rd and 4th nucleotides after PAM. Deletion junction sequences of 7 founders, #1, #9, #11, #15, #20, #30 and #35; duplication junction sequences of two founders, # 1 and #20; INV-R (right) junction sequences of four founders: #2, #10, # 21, and #39; INV-L (left) junction sequences of four founders: #2, #10, # 21, and #39