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Fig. 4 | BMC Genetics

Fig. 4

From: Maternal variant in the upstream of FOXP3 gene on the X chromosome is associated with recurrent infertility in Japanese Black cattle

Fig. 4

The upstream region of FOXP3 including a SNP (g.92,377,635A > G) affects FOXP3 expression. Luciferase reporter assays for the 5′ upstream region of FOXP3-derived risk- and non-risk-alleles. The 5′ upstream region (2364-bp fragment upstream, 92,375,328–92,377,691 bp) derived from the non-risk- and risk-alleles were cloned into the firefly luciferase pGL3-non-risk and pGL3-risk plasmids, respectively. The Firefly-to-Renilla luminescence ratios observed after cotransfecting HEK293T cells were measured to evaluate the effects of the 5′ upstream region. Mock is a backbone plasmid without Luciferase gene of pGL3-basic plasmid. Bars represent the mean ± standard error obtained in triplicate from each of three independent experiments. P values determined by t tests are shown

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