TY - JOUR AU - Igwe, David Okeh AU - Afiukwa, Celestine Azubike AU - Ubi, Benjamin Ewa AU - Ogbu, Kenneth Idika AU - Ojuederie, Omena Bernard AU - Ude, George Nkem PY - 2017 DA - 2017/11/17 TI - Assessment of genetic diversity in Vigna unguiculata L. (Walp) accessions using inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) polymorphic markers JO - BMC Genetics SP - 98 VL - 18 IS - 1 AB - Assessment of genetic diversity of Vigna unguiculata (L.) Walp (cowpea) accessions using informative molecular markers is imperative for their genetic improvement and conservation. Use of efficacious molecular markers to obtain the required knowledge of the genetic diversity within the local and regional germplasm collections can enhance the overall effectiveness of cowpea improvement programs, hence, the comparative assessment of Inter-simple sequence repeat (ISSR) and Start codon targeted (SCoT) markers in genetic diversity of V. unguiculata accessions from different regions in Nigeria. Comparative analysis of the genetic diversity of eighteen accessions from different locations in Nigeria was investigated using ISSR and SCoT markers. DNA extraction was done using Zymogen Kit according to its manufacturer’s instructions followed by amplifications with ISSR and SCoT and agarose gel electrophoresis. The reproducible bands were scored for analyses of dendrograms, principal component analysis, genetic diversity, allele frequency, polymorphic information content, and population structure. SN - 1471-2156 UR - https://doi.org/10.1186/s12863-017-0567-6 DO - 10.1186/s12863-017-0567-6 ID - Igwe2017 ER -