Skip to main content
Fig. 3 | BMC Genetics

Fig. 3

From: Gene expression patterns of chicken neuregulin 3 in association with copy number variation and frameshift deletion

Fig. 3

Sequence homology between chicken NRG3 and duplicated segment in red junglefowl reference genome (RJF; JH375293). a Primers for quantitative PCR (qPCR) were designed in the regions where these 2 sequences are the least conserved (shown in gray highlights). The relative copy number of this duplicated segment was estimated by qPCR between chicken breeds as well as NRG3 genotypes. Sequence similarities are shown in curly brackets with length. b Hinai-jidori (HJ) has a significantly higher number of copies than the other breeds (Dunn’s multiple comparisons test: P < 0.001). c, d Boris Brown (BB) and HJ display different patterns of copy number gains. In HJ, the relative copy number is different between the groups with the wild-type (WT) allele and with the putative frameshift deletion (del) in chicken NRG3 (Mann–Whitney U-test: P < 0.05). The number in parentheses shows individuals from each group. Vertical bars represent the standard error of the mean

Back to article page