Fig. 2

Sequencing of homozygous ewes carriers and non-carriers of the FecX ^Bar allele. a Sanger sequencing of the end of first exon of the ovine BMP15 gene. Nucleotide numbering and amino-acid translation are relative to the ATG start site. The FecX ^Bar variant haplotype (Bar/Bar) is indicated within green boxes and compared to wild-type haplotype (+/+). b Protein alignment between reference ovine BMP15 and Barbarine mutated BMP15^A101CfsX113 translated from homozygous FecX ^Bar variant. The 112 alternative amino-acids generated by the frameshift (fs) are highlighted in green. Views were obtained from CLC MainWorkbench 7.6.4 (Qiagen Aarhus)