Fig. 4

Overview of candidate genes for interaction partners of DmManf based on RNAi screen. UAS-x-RNAi lines were crossed to MS1096-GAL4 and 69B-GAL4 driver lines in wild type, heterozygous DmManf ^Δ96 mutant and DmManf overexpression background. Observed phenotypes of knockdown flies in heterozygous DmManf ^Δ96 mutant background (het vs. wt) and DmManf overexpression background (OE vs. wt) were compared to phenotype of knockdown flies in wild type background. Yellow (stronger phenotype) and green (rescued phenotype) represent affected phenotypes. Light gray (no phenotype), gray (phenotype not affected) and dark gray (lethal phenotype) represent cases where heterozygous DmManf ^Δ96 mutant background or overexpression of DmManf did not affect the phenotype caused by knockdown of target gene. For a comparison, results from microarray analysis (MAA) [14] are presented; red and blue indicate up- and downregulation of target genes, respectively. Mutant larvae stands for zygotic DmManf ^Δ96 mutant larvae, OE larvae for 69B-GAL4 > UAS-DmManf ^L3 larvae, and mutant embryos for maternal and zygotic DmManf ^Δ96 mutant embryos. Cellular compartment: Ch, chromosome; Cy, cytoplasm; Ec, extracellular; Me, membrane; Mi, mitochondrion; Nu, nucleus; nd, no data available; Sg, signalosome; Sp, spliceosome. Het, heterozygous DmManf mutant; MAA, microarray analysis; OE, overexpression