Table 1 Primers used to amplify the TYR gene, the D-loop and Cytochrome b
Gene | Primer | Annealing temperature | Nucleotide sequence |
|---|---|---|---|
1TYR Exon 1 | TYR11Fw | 59 | 5' CCAATTAGCCAGTTCCTGCAGA 3' |
TYR11Rv | 5' CACAGTTGAATCCCATGAAGTTGC 3' | ||
TYR12Fw | 57 | 5' TATAATAGGACCTGCCAGTGCTCTG 3' | |
Tyr12Rv | 5' AATGTCTCTCAAGATTTCAGATCCC 3' | ||
Tyr13Fw | 60 | 5' TGTGTCAATGGATGCACTGCTT 3' | |
Tyr13Rv | 5' AGAAGTGATTGTTAAGGTTCCTCCC 3' | ||
1TYR Exon 2 | Tyr2Fw | 60 | 5' TTGTTTAACATGAGGGTGTTTTGTACAG 3' |
Tyr2Rv | 5' GGACTTTGGATAAGAGACTGTAAATATG 3' | ||
TYR Exon 3 | Tyr3SbFw | 59 | 5' TCCATTTACTGGGATAGCAGATG 3' |
Tyr3SbRv | 5' GTGAAGAAGGAAGATGGGATCAT 3' | ||
TYR Exon 4 | Tyr4SbFw | 59 | 5' GAAGGCATCGCCCTCTTCTA 3' |
Tyr4SbRv | 5' AGGTAGCTATAGTCATAGCCCAGA 3' | ||
TYR Exon 5 | Tyr5SbFw | 60 | 5' CCCAGACTCTTTTCAAGACTAACATT 3' |
Tyr5SbRv | 5' AATAAAGATGGGGCCAATAAAAA 3' | ||
2D-loop | L | 60 | 5' GGCCTTGTAAACCGGAAAAGG 3' |
R | 5' GAAAGGCTAGGACCAAACCTG 3' | ||
3CytB | L14724 | 58 | 5' CGAAGCTTGATATGAAAAACCATCGTTG 3' |
CIT-REV | 5' GAATATCAGCTTTGG 3' |