A novel nonsense mutation in the tyrosinase gene is related to the albinism in a capuchin monkey ( Sapajus apella )

Table 1 Primers used to amplify the TYR gene, the D-loop and Cytochrome b

Gene	Primer	Annealing temperature	Nucleotide sequence
¹TYR Exon 1	TYR11Fw	59	5' CCAATTAGCCAGTTCCTGCAGA 3'
	TYR11Rv	59	5' CACAGTTGAATCCCATGAAGTTGC 3'
	TYR12Fw	57	5' TATAATAGGACCTGCCAGTGCTCTG 3'
	Tyr12Rv	57	5' AATGTCTCTCAAGATTTCAGATCCC 3'
	Tyr13Fw	60	5' TGTGTCAATGGATGCACTGCTT 3'
	Tyr13Rv	60	5' AGAAGTGATTGTTAAGGTTCCTCCC 3'
¹TYR Exon 2	Tyr2Fw	60	5' TTGTTTAACATGAGGGTGTTTTGTACAG 3'
¹TYR Exon 2	Tyr2Rv	60	5' GGACTTTGGATAAGAGACTGTAAATATG 3'
TYR Exon 3	Tyr3SbFw	59	5' TCCATTTACTGGGATAGCAGATG 3'
TYR Exon 3	Tyr3SbRv	59	5' GTGAAGAAGGAAGATGGGATCAT 3'
TYR Exon 4	Tyr4SbFw	59	5' GAAGGCATCGCCCTCTTCTA 3'
TYR Exon 4	Tyr4SbRv	59	5' AGGTAGCTATAGTCATAGCCCAGA 3'
TYR Exon 5	Tyr5SbFw	60	5' CCCAGACTCTTTTCAAGACTAACATT 3'
TYR Exon 5	Tyr5SbRv	60	5' AATAAAGATGGGGCCAATAAAAA 3'
²D-loop	L	60	5' GGCCTTGTAAACCGGAAAAGG 3'
²D-loop	R	60	5' GAAAGGCTAGGACCAAACCTG 3'
³CytB	L14724	58	5' CGAAGCTTGATATGAAAAACCATCGTTG 3'
³CytB	CIT-REV	58	5' GAATATCAGCTTTGG 3'

ISSN: 2730-6844