Table 5 Newly developed SSR markers for qPH.A-1.3 region
Marker | Forward primer(5'-3') | Reverse primer(5'-3') | Annealing temperature (°C) |
|---|---|---|---|
MPH72 | CTGGGAAGGAAACCTAAACA | CGACTGAGGACACCTATAGACA | 58 |
MPH96 | GTTGCCTTGTTCTTGATTCAC | TAGCTGCCAGTGGTACTTTTC | 60 |
MPH1056 | TATCCGCTTTCTTCCCTTCT | ACCGCAACCATTCAACATAC | 58 |
MPH1042 | CCGCTTTCTTCCCTTCTCTT | CCGCAACCATTCAACATACA | 58 |
MPH1061 | CGCGTAAGTTGTGTGTTTTT | TCTTTTAGTTGAGGCCATTC | 57 |
MPH1088 | GCACGCAAGAGAGGAATAGA | AAGAGGGAGGATGAGGATTA | 58 |
MPH16 | AGGAGCTAGGGATTGAATATG | GAATTTGACCCGAATTTCC | 58 |
MPH1132 | CCTGTCAGAGACAGTTCTC | GAGAGAAGAAAAGGGGTACG | 59 |
MPH1149 | GAACATACCAGTATGGAAGGA | GACCAAATTGGGACTTAACC | 58 |
MPH5324 | TCCAAGTGACAGAATAAACTTTC | ATCACAAGGGTCATCTTCCG | 58 |