Fig. 6From: Identification of effector-like proteins in Trichoderma spp. and role of a hydrophobin in the plant-fungus interaction and mycoparasitism tvhydii1 knockout and overexpressing strains. a confirmation of the tvhydii1 gene deletion; lane 1, 5’ end of the deletion cassette inserted at the tvhydii1 locus; lane 2, tvhydii1 wild type amplification as control; lane 3, tvhydii1 amplification in the knockout strains; lane 4, 3’ end of the deletion cassette inserted at the tvhydii1 locus. b semi-quantitative RT-PCR of the knockout and overexpressing strains; RT-PCR reactions were carried out using gpd gene as amplification control for tvhydii1 gene; c hydrophobicity assay, a 20 μL drop of distilled water was placed over plugs of 72 h mycelia of each T. virens strainBack to article page