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Fig. 4 | BMC Genetics

Fig. 4

From: Protanopia (red color-blindness) in medaka: a simple system for producing color-blind fish and testing their spectral sensitivity

Fig. 4

The equipment used for the O-O test. (a) and (b) Layout and appearance of the rotary-cylinder device. A glass aquarium was placed at the center of a rotary cylinder with vertical black-white stripes on the inner wall (in this picture, the stripes consist of aluminum-foil strips on Indian black ink for experiments under ultraviolet light; see text). The cylinder can be rotated by an electric motor at a constant speed (6 rpm). (c) Layout of the experimental platform for irradiation and behavior recording during the O-O test. The horizontal monochromatic light (ML) is reflected on the first mirror (mirror1) to illuminate the rotary-cylinder device from the top. The light goes through the aquarium, a white plastic paper under the aquarium, and the transparent acrylic base of the rotary-cylinder device (not shown), and this transmitted light (TL) is then horizontally reflected by the second mirror (mirror2), which is recorded by a digital CCD camera (ORCA-R2; Hamamatsu Photonics). The monochromatic lights are parallel lights and show the shadows of the fish in the aquarium clearly on the plastic paper (see Fig. 5), which we observed for the behavioral analyses. A part of the monochromatic light is scattered by the plastic paper, and these scattering lights (SL) can irradiate the inside of the cylinder. (d) and (e) Actual appearance of the experimental platform under the ceiling lights in the OLS room (d) and monochromatic red light (e). A flashlight was also used when taking these pictures

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