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Fig. 1 | BMC Genetics

Fig. 1

From: Protanopia (red color-blindness) in medaka: a simple system for producing color-blind fish and testing their spectral sensitivity

Fig. 1

Detailed schemes for introducing double-ins/del mutations into the LWSa and LWSb loci and a summary of the results. (a) Genomic structures of the LWSa and LWSb loci. Coding exons are shown as solid boxes. (b) Alignment of the second exons of the paralogous LWS sequences. The A residue in the first exon, at which translation is initiated, is numbered as the first. Shaded white text indicates identical nucleotides. Only four nucleotides were different between the LWS genes. Red, yellow, green, and blue text indicate the target sequences of the sgRNA-1, 2, 3, and 4, respectively. (c) Details and efficiencies of inducing the double-ins/del mutations on the LWSa and LWSb genes in the G0 generation. The sgRNA-4 achieved the highest induction efficiencies in both the embryonic and adult stages (boxed)

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