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Table 2 List of all of MHC IIB alleles found in the Midas cichlid

From: Molecular characterization of MHC class IIB genes of sympatric Neotropical cichlids

Samples AM05 AM06 AH65 AL11 AL64 AQ72 27D3 25D8 27G4 25A2 25D1 25B2 27E7 Total Sequences
Template Allele gDNA gDNA gDNA gDNA gDNA gDNA gDNA cDNA gDNA cDNA cDNA gDNA cDNA gDNA cDNA gDNA cDNA gDNA cDNA
Amci-DXB*000101 12 13 69 3   21 46              164
Amci-DXB*000102 71 10 49   13 77 4              224
Amci-DXB*000103 1      1               2
Amci-DXB*000104 1    1                 2
Amci-DXB*000105 1   1                  2
Amci-DXB*000106   1     2               3
Amci-DXB*000107    1    1               2
Amci-DXB*000108    1    1               2
Amci-DXB*0002 1     1                2
Amci-DXB*0003 1   1          1         3
Amci-DXB*0004    1 1   1 13              16
Amci-DXB*0005        2              2
Amci-DXB*0006    2    1 2              5
Amci-DXB*0007 1     1                2
Amci-DXB*0008 2 1    1                4
Amci-DXB*0009    1    1               2
Amci-DXB*0010     1   1               2
Amci-DXB*0011   1 1                  2
Amci-DXB*0012    1    1 1              3
Amci-DXB*0013    1 1                 2
Amci-DXB*0101    2 2   3 1   2            10
Amci-DXB*0102 5   2 2 1 9               19
Amci-DXB*0201        39      1     4     44
Amci-DXB*0202        17      14     26 1    58
Amci-DXB*0203          6    6         12
Amci-DXB*03             26     1     27
Amci-DXB*040101 9 1 4   2 13 7   4    9     10   2   61
Amci-DXB*040102 5 1 5 3 1       17     3   8    43
Amci-DXB*040103 1      1               2
Amci-DXB*0402 1      1               2
Amci-DXB*040301     3 2 1   1   25      3   7    42
Amci-DXB*040302    1    1               2
Amci-DXB*040303 2      16 13              31
Amci-DXB*040304     3 8   18              29
Amci-DXB*040305             4         4
Amci-DXB*0404 2      1               3
Amci-DXB*0405        3 26     1    16   20    66
Amci-DXB*0406         1        1      2
Amci-DXB*05    1                  1
Amci-DXB*060101         1      43 1 31 2 42   16 136
Amci-DXB*060102              1   1      2
Amci-DXB*0602              1   6      7
Amci-DXB*0603           5          3 8
Amci-DXB*0604   1              1     16 18
Amci-DXB*07              2        2
Amci-DXB*08               1 40      41
Amci-DXB*09           50          9 59
Amci-DXB*1001                1     25 26
Amci-DXB*1002                1      1
Amci-DXB*110101        31 42             73
Amci-DXB*110102         2             2
Amci-DXB*1102         8             8
Amci-DXB*12           1      19      20
Amci-DXB*13            1          1
Amci-DXB*14                    38 38
Amci-DXB*15           16           16
Amci-DXB*16        4         34      38
Amci-DXB*17        4 18             22
Amci-DXB*18             17 44    23 50    134
Amci-DXB*19                  9    9
Amci-DXB*20                    2 2
Amci-DXB*21                2      2
Amci-DXB*2201        2 23             25
Amci-DXB*2202         2             2
Amci-DXB*23         2             2
Amci-DXB*2401         13             13
Amci-DXB*25             1     3     4
Amci-DXB*26           6           6
Amci-DXB*27 2                    2
Total sequences 118 29 144 20 30 154 207 139 12 103 18 80 91 2 159 69 137 2 109 1623
Total Alleles 17 8 18 10 9 20 25 9 2 14 14 11 8 69
  1. Alleles found in 13 Midas cichlid samples (listed on the top) showing number of copies recovered per allele amplified in gDNA and cDNA in each individual. All alleles except DXB*05, DXB*1002, DXB*0402, and DXB*13 were confirmed with at least two independent PCR reactions. At the bottom we summarize total number of sequences and total number of alleles obtained