Table 1 Primer combinations used for amplification of MHC IIB
From: Molecular characterization of MHC class IIB genes of sympatric Neotropical cichlids
Forward | Primer Reverse Primer | TA | Fragment |
|---|---|---|---|
SatoGa11_mod1 | MHC Rev3 | 59 °C | E2 - E3 |
 AACTCCACKGAKCTGAAGRAC |  GATCTGTTTGGGGTAGAAGTCG |  |  |
GAIIEx2startF | MHC Rev3 | 55 °C | E2 - E3 |
 GTCTTTAACTCCACGGAGCTGAAGG |  GATCTGTTTGGGGTAGAAGTCG |  |  |
GAIIEx2startF | AcMHCIIBR1 | 59 °C | E2 - E3 |
 GTCTTTAACTCCACGGAGCTGAAGG |  GGRGTGAAGTCTGACTRATGG |  |  |
AaMHCIIBE1F1b | MHC Rev3 | 56 °C | E1 - E3 |
 ATGGCTYCATCCTTYMTC |  GATCTGTTTGGGGTAGAAGTCG |  |  |
AaMHCIIBE1F1b | AcMHCIIBR4 | 59 °C | E1 - E5 |
 ATGGCTYCATCCTTYMTC |  ACCCAGGATCAGTCCTGAGG |  |  |
AcMHCIIBF2 (excludes group I) | AcMHCIIBR3 (excludes gop I) | 64 °C | E2 - E2/I2 |
 TTAACTCCACKGAGCTGAASGACA |  GAYGATGAAYCATAACTCACCTGAT |  |  |
AcMHCIIBF3 (only group I) | AcMHCIIBR4 | 46 °C | E2/I2 - E5 |
 TCAGGTGAGTYATGDTTCATC |  ACCCAGGATCAGTCCTGAGG |  |  |
AcMHCIIBF3 (only group I) | AcMHCIIBR5 | 44 °C | E2/I2 - R5 |
 TCAGGTGAGTYATGDTTCATC |  TTCCTCTTGTAGTAGATGAATCC |  |  |
AcMHCIIBF4 (excludes goup I) | AcMHCIIBR4 | 54 °C | E2/I2 - E5 |
 TCAGGTGAGTCTGTTTCTGTG |  ACCCAGGATCAGTCCTGAGG |  |  |
AcMHCIIBF5 (excludes group I) | MHC Rev3 | 55 °C | E2 - E3 |
 CCACKGAGCTGAASGACATSGAG |  GATCTGTTTGGGGTAGAAGTCG |  |  |
AcMHCIIBF5 (excludes group I) | AcMHCIIBR4 | 59 °C | E2 - E5 |
 CCACKGAGCTGAASGACATSGAG |  ACCCAGGATCAGTCCTGAGG |  |  |
AcMHCIIBF7 | AcMHCIIBR9 | 50 °C | E2 - E2 |
 CGAGTACGTTCGATCTTTGTATTGC |  DCTGATTTAGTCAGAGCAGTCT |  |  |
AcMHCIIBF8 | AcMHCIIBR9 | 55 °C | E2 - E2 |
 CGAGTWCATCARVTCTTACTAYTWC |  DCTGATTTAGTCAGAGCAGTCT |  |  |
AcMHCIIBF9 | AcMHCIIBR8 | NA | I2 - I2 |
 GAAACCTGTTCACAGCAGTCCCTC |  CATGTGCTACATGCAACATATCA |  |  |
AcMHCIIBF6 | MHC Rev3 | 55 °C | E2 - E3 |
 CCACTGAGCTGAASGACATSGAG |  GATCTGTTTGGGGTAGAAGTCG |  |  |