Table 1 Primer combinations used for amplification of MHC IIB
From: Molecular characterization of MHC class IIB genes of sympatric Neotropical cichlids
| Forward | Primer Reverse Primer | TA | Fragment |
|---|---|---|---|
| SatoGa11_mod1 | MHC Rev3 | 59 °C | E2 - E3 |
| AACTCCACKGAKCTGAAGRAC | GATCTGTTTGGGGTAGAAGTCG | ||
| GAIIEx2startF | MHC Rev3 | 55 °C | E2 - E3 |
| GTCTTTAACTCCACGGAGCTGAAGG | GATCTGTTTGGGGTAGAAGTCG | ||
| GAIIEx2startF | AcMHCIIBR1 | 59 °C | E2 - E3 |
| GTCTTTAACTCCACGGAGCTGAAGG | GGRGTGAAGTCTGACTRATGG | ||
| AaMHCIIBE1F1b | MHC Rev3 | 56 °C | E1 - E3 |
| ATGGCTYCATCCTTYMTC | GATCTGTTTGGGGTAGAAGTCG | ||
| AaMHCIIBE1F1b | AcMHCIIBR4 | 59 °C | E1 - E5 |
| ATGGCTYCATCCTTYMTC | ACCCAGGATCAGTCCTGAGG | ||
| AcMHCIIBF2 (excludes group I) | AcMHCIIBR3 (excludes gop I) | 64 °C | E2 - E2/I2 |
| TTAACTCCACKGAGCTGAASGACA | GAYGATGAAYCATAACTCACCTGAT | ||
| AcMHCIIBF3 (only group I) | AcMHCIIBR4 | 46 °C | E2/I2 - E5 |
| TCAGGTGAGTYATGDTTCATC | ACCCAGGATCAGTCCTGAGG | ||
| AcMHCIIBF3 (only group I) | AcMHCIIBR5 | 44 °C | E2/I2 - R5 |
| TCAGGTGAGTYATGDTTCATC | TTCCTCTTGTAGTAGATGAATCC | ||
| AcMHCIIBF4 (excludes goup I) | AcMHCIIBR4 | 54 °C | E2/I2 - E5 |
| TCAGGTGAGTCTGTTTCTGTG | ACCCAGGATCAGTCCTGAGG | ||
| AcMHCIIBF5 (excludes group I) | MHC Rev3 | 55 °C | E2 - E3 |
| CCACKGAGCTGAASGACATSGAG | GATCTGTTTGGGGTAGAAGTCG | ||
| AcMHCIIBF5 (excludes group I) | AcMHCIIBR4 | 59 °C | E2 - E5 |
| CCACKGAGCTGAASGACATSGAG | ACCCAGGATCAGTCCTGAGG | ||
| AcMHCIIBF7 | AcMHCIIBR9 | 50 °C | E2 - E2 |
| CGAGTACGTTCGATCTTTGTATTGC | DCTGATTTAGTCAGAGCAGTCT | ||
| AcMHCIIBF8 | AcMHCIIBR9 | 55 °C | E2 - E2 |
| CGAGTWCATCARVTCTTACTAYTWC | DCTGATTTAGTCAGAGCAGTCT | ||
| AcMHCIIBF9 | AcMHCIIBR8 | NA | I2 - I2 |
| GAAACCTGTTCACAGCAGTCCCTC | CATGTGCTACATGCAACATATCA | ||
| AcMHCIIBF6 | MHC Rev3 | 55 °C | E2 - E3 |
| CCACTGAGCTGAASGACATSGAG | GATCTGTTTGGGGTAGAAGTCG |