Fig. 5

CAN1 mutations in JC5516 are greatly elevated by DNA replication stress. Mean and standard deviation for CAN1 (a) or URA3 (b) mutation rates in JC5516 grown for three days at 20 °C (or four days for HU and MMS) in standard rich YPD medium (Control), in YPD with chronic exposure to 10 mM acetic acid, 30 mM HU, or 200 μM MMS, or in three rad52∆ derivatives grown in YPD. Control and MMS data are the same as shown in Fig. 4. Results of three to four trials are shown for treatments and rad52∆ mutants. Double asterisks indicate p < 0.01 versus control