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Fig. 3 | BMC Genetics

Fig. 3

From: Formation of diploid and triploid hybrid groupers (hybridization of Epinephelus coioides ♀ × Epinephelus lanceolatus ♂) and their 5S gene analysis

Fig. 3

Histological sections of early embryo development of E.coioides. a 30 s after fertilization (AF), white arrows mean the sperm aster, which was framed in the white box and enlarged beside; b 1 min AF, white arrow indicated sister chromatids in the second meiosis metaphase stage; c 2 min AF, white arrow indicated the separating sister chromatids in the second meiosis anaphase; d 4 min AF, the second meiosis telophase, white arrow indicated the second polar nuclear; e 8 min AF, white arrows indicated the fusion of the two pronuclei, which were framed in the white box and enlarged beside; f 16 min AF, white arrow indicated the replication bubblo; g 20 min AF, white arrow indicated the replication bubblo; h 24 min AF, white arrow indicated metaphase chromosome; i 28 min AF, white arrow indicated the divorcing chromosomes; j 36 min AF, white arrow indicated the replication bubblo; k 40 min AF. Bars = 20 μm

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