Table 2 SSR primer sequences used for amplification in 32 O. viciifolia individuals and characteristics of SSR motifs
From: Characterization of novel SSR markers in diverse sainfoin (Onobrychis viciifolia) germplasm
Marker | Motif | Repeats | Predicted size | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|---|---|---|
OVK002 | AG | 9 | 164 | CCCACCAGACAAAAAGAATA | GCTTTCCCCTTCATCAACTAT |
OVK003 | TA | 8 | 122 | GATAGAATTCGTTTGTTGGTG | ATCTTTGTAACTGTTCGCTCA |
OVK017 | AC | 8 | 158 | GGGTGTTAGTTATCCATTTCC | ACATACTAGCCTTCTGGGGTA |
OVK027 | CTCG | 6 | 129 | AATGGAATCTCGGAGACAG | GGAAGAAGACGAAGTAGTAGGA |
OVK034 | GCT | 6 | 150 | GTGAGATGAGCTTGGACATT | AGATAACTAACTGCAGGCAAG |
OVK036 | AGGT | 6 | 150 | GTGTTAAAGGGGTGAAAACAT | CATTTTGACAAACCAGTATCC |
OVK038 | ATT | 6 | 166 | CCACATACGAGACAGAATAGG | CTGAAAATTGATCGATACTGG |
OVK042 | GTT | 6 | 144 | GGAACGGTTAATTTCTGATTT | AGAATTCCGTACAAGTCGAG |
OVK045 | AGA | 6 | 148 | CCAAAAATCATCAATCAACAC | TTGAACAAGGGTTAGGGTTAT |
OVK046 | AGTG | 6 | 151 | TCAACCACATTATAAAACCTCA | CGCGAAATCATAGTTCACTT |
OVK054 | GAA | 6 | 201 | TTGCAGAGATAACACTCACCT | TCCTGAAAAACCTAATCACAA |
OVK055 | GAT | 6 | 189 | GAAGATATTTCAAAGCAGCAA | CATGCTACCACTAGCAGAAGT |
OVK063 | TTG | 6 | 188 | AATTGCAACTGAAACTGAAAC | ACTGCTACCCTCTCCATAAAT |
OVK068 | GGA | 6 | 195 | GACCACCCGCAGCTCAAC | GTCTTCTTCCCCCATATTTAG |
OVK072 | ACC | 7 | 199 | TTGCCTTAGTCAGTTACCTTG | GTGGAGAGAATGAGAGAACCT |
OVK073 | GAC | 6 | 200 | GTAGACAACCGTATCTGGACA | AAGATGGAAGGTTCTAGTTCG |
OVK077 | TTA | 6 | 249 | GTCCCTCTCTCTCAAATTGTT | AGGTTAATGGAGCTTAGTGGT |
OVK089 | CAT | 6 | 257 | CAAAGTCATACCAATCACCAT | TCTTGGAAGCACTTGTTACTC |
OVK093 | CCA | 6 | 259 | CCAAGTGTTTGAAAGTCTCAG | TGAGAGTTCGTTCAAGGTAGA |
OVK094 | TTGCG | 5 | 255 | ACCGATCTTAGGATAGATGGA | ACTTTTGGTTGCTTAGTCGAT |
OVK096 | TCA | 6 | 249 | GAGCGTTGCATTTACATTTAC | CATCCTCCTTTACACCCTAAT |
OVK097 | GTGA | 6 | 252 | TCTATAGAGATGAGGCGACAA | CGCCCCTAACTAACCTACTAC |
OVK099 | TGAG | 6 | 247 | AGAAAATGGAAGCAACAGAGT | ACAAATAGCAGCTCCCTTC |
OVK101 | CTAA | 6 | 254 | GTTGAGTTTCAGACACAGAGC | AATAGCTCCCACAATAACTCC |
OVK102 | TGT | 6 | 249 | CCAAAGGGTGTTTTATTTTCT | GGAAGAAATTAAGCAAATGGT |
OVK107 | AG | 8 | 193 | AAGTTAAAACTTTGCGTTGTG | GACGTTGTTCTGGATTTCTTC |
OVK111 | GGT | 7 | 206 | TATAGACCTTCTCCTCCCAGA | GTGAAAGTCACAAATCCAAAG |
OVK119 | CAG | 6 | 199 | ACCCTCCTTCTCTCCTTATTT | GACGAGAGAACTCGTTTATGA |
OVK122 | TC | 9 | 211 | GCAGATAGCACAGTTATCGAC | GAACCACACACACAGAATCA |
OVK123 | ACA | 9 | 200 | CACCCATTAACTATCATGGTC | CAAGCCCTTTGTGAGATACTA |
OVK124 | TGA | 9 | 211 | GCCTTTTCTGTGACTCGTAA | GCTCCATTCCCATTTATAGTT |
OVK125 | CATTT | 5 | 193 | AAATTTAAGCACCGGAATAAC | AAAGCAAAAGGGCTACTAAAG |
OVK126 | TC | 8 | 197 | CGACAAAACTATTTAGGCAAA | GGGAAGAGATCATAAACCCTA |
OVK127 | AT | 8 | 200 | GCCCAAAATGTATTATCCTTC | AGAACAGACAGATATGCAAGC |
OVK131 | TA | 8 | 200 | TCTATCTGGGTGTTGTTTTGT | CTGTTTGAATATCGATTACCAC |
OVK133 | TG | 8 | 196 | TGCTTCAGCATTATTGTAACAT | TGCACTTCTCCATACTTCCTA |
OVK138 | CTAA | 6 | 250 | TAATATGGTGCAAGTTCCAAT | TTCTACGCTTAGCTCAAACC |
OVK141 | CACG | 6 | 239 | GAGGAGGTACATACAGCACAG | CAACCTCCTCGTTATCTTTTT |
OVK142 | GT | 8 | 243 | AACATGACTACTGTGAACAAGG | CGAACATGTAATTGATCCAAG |
OVK155 | GTG | 6 | 251 | CAGGTTTGAAGTAGCAGAGAA | GTAGACCACGCATACTGAATC |
OVK158 | GACT | 6 | 257 | TCAGAGTGTGTTGTGTTGTGT | AGTGAAGCAAATGTGTGATTT |
OVK159 | TG | 8 | 251 | CATTATTGCCTAGCATTGTTC | ATTTCACCATCAAGTATGCAC |
OVK161 | TTCC | 6 | 249 | AAAGCTTTCTACACGTTGGTA | TGGGTTTTTACACTCTGTGAT |
OVK165 | ACA | 6 | 267 | TTTCAAACACTCACTCACTCC | TCGGATTTGTGACCTAACTC |
OVK168 | TGA | 6 | 253 | AATTATCACCCACTGCTATGA | GGTTTCCATCACTGTTTGTTA |
OVK172 | AGC | 6 | 256 | TTATTAAACCTGCGTCTTCTG | GTAGAGCTGTGGGCTTTATCT |
OVK173 | CT | 8 | 253 | TCGTTCTCGTGATTATTCTGT | CCTCTATTCAAATAGGGCAAT |
OVK174 | GGCCC | 5 | 246 | ACATGATCGTGAATATGAAGC | CAGCAGCAATCAATATATCATC |
OVK175 | CA | 9 | 250 | GTAAAATATCAAGCAGGAGCA | AAACTATGCAGACACCCTGTA |
OVK177 | CTG | 7 | 257 | TCTGTTGATTTAAGGAGACGA | CTCTTGCTCATATTTTCCTCA |
OVK181 | AAG | 6 | 257 | AGGAAGAAGAAGAAGAAGCAG | TTCTCCTTTAACCACAACCTT |
OVK183 | TGAT | 6 | 256 | GAGGGTAAGAGAGAGTGGAAG | CTTGCCTGATATCTTCTCAAA |
OVK196 | AGC | 6 | 286 | TTTTGAGAGTGTGGAAGGTTA | AGTATGAGCCTGATGATGATG |
OVM003 | TC | 9 | 297 | CCGTCTGTTTAATCATTCACT | GAAAGGAAAGGTTATTGGAGA |
OVM004 | ATTT | 5 | 290 | GGGAATTCTTAAATCTCATGG | ATGCATGGTACTGGGTCGT |
OVM025 | CAA | 6 | 297 | TTCTGAACAACAACAACAACA | GTCCAGGAGCTAAGTAACCAT |
OVM031 | TGA | 6 | 306 | ATTGGTTTCTAAGGAGGACTG | GCAATACTCCTCTGCCTAGTT |
OVM033 | CTC | 6 | 300 | CAAGGCTTATTTGGTTAACAG | ATACTATTTCCCATGCCTACC |
OVM034 | TTC | 6 | 308 | GCATTTCATCAAACACTTTTC | TTGGTTTGAATCTGTGAGACT |
OVM035 | TTC | 7 | 303 | TCATCAAACACTTTTCGTTCT | TTGGTTTGAATCTGTGAGACT |
OVM038 | GAAG | 6 | 297 | CACAGGACAAGAGTGAGAGAG | TCATGATACCACGAATTTTTC |
OVM043 | GAG | 7 | 167 | TAGTATGGCTGAAATCAAAGG | ATATCATAAGGGCAACAGTGA |
OVM048 | AT | 9 | 157 | GACATTGAAATCAAACAATCC | AACACTTGTCATGTTTCCAAG |
OVM049 | TGA | 7 | 150 | AACAAACAAGAGGAAAAGGAG | TATGTGCTTATCAGGCATTTT |
OVM050 | ATCC | 6 | 161 | ATGAGCATGAAGAGTTTCAGA | ACACATCTACGACTTCTTTCG |
OVM053 | GTGGA | 5 | 149 | CACCAAAAGCATAGCAATAGT | GCTTGAATTGAATGAGAAATG |
OVM057 | TTG | 6 | 153 | CCTTGAGGAGGAATAATAGGA | GACATCATCATCACCTTCACT |
OVM058 | AT | 9 | 150 | GTCAAGTCATACCCATACGAG | CAGTGTAACCATATGCACAAA |
OVM059 | AGA | 6 | 149 | ACTCCAACTCCAACTCAGAAC | AAGCGAAGAAGAGAGTGAGAT |
OVM060 | CT | 8 | 159 | ATGTAATCAAAAGGTGCAGAA | AGCTTCCAAAACAGTGTATGA |
OVM061 | GTA | 6 | 150 | TTAACACACGTACGTACCACA | TTTGTCGTTGATCGTTAAGTT |
OVM062 | AG | 8 | 139 | GGAAAAAGGTTTGGATAGATG | AAGTTTTCCCCACACTATTCT |
OVM064 | AT | 8 | 353 | GCATGCACAGAATTAAGTTTC | AGAAGGTCCTTTGAAAATCAG |
OVM065 | CT | 8 | 352 | AAGACAGCGAGTTACCAATCT | GATTGAAACTGAGTAGCGATG |
OVM067 | CTT | 6 | 352 | CAACCTTAATACCAACCTTCC | AAAAGTAGCCAGAGAGCAAAT |
OVM068 | CCT | 6 | 333 | CTACAACTCACCGAAACTCAC | CGATTTCTGCCTCTTTATTCT |
OVM069 | AATG | 6 | 357 | ATGTTGTACAGATGAGCTTCG | TAGTGAGCAAACCTATTTTGG |
OVM072 | GAA | 6 | 350 | TTGATGTGGTTGATCCTATTC | GATGTCAACATCTTGGTCATTA |
OVM073 | ACA | 6 | 346 | GTTCTCAAACGCACTATCAAC | AAAATCTTGTAGGGATTCGAT |
OVM076 | AAC | 7 | 348 | CCCATTCTTCATCTTTCTCTC | TGCTTCCATAATCAGTGAAAT |
OVM081 | GT | 9 | 350 | TCTAGCACAATGTTTTGGATT | TATTGAGTTGAAGCAGACCAT |
OVM083 | CT | 8 | 347 | CACACAAACACAAAACTCACA | GATCGGAGAAAAGAAGAAGAG |
OVM086 | GAA | 6 | 350 | TCATACAAAGTTCCTTCCGTA | ATTGCCAATAACAGTGAAGAG |
OVM090 | CCA | 6 | 151 | AATCAATGGAGGAGGATAAAC | GAAGGTTGAAAAGGGAATAAA |
OVM091 | ATC | 6 | 188 | AACCACCCTTAATTCCATAAG | AGATAAAAGCCGCAAAAGTAT |
OVM092 | CAC | 6 | 157 | GGACCAACAAAGAGGATTATT | CCCTTGCTTGAAGTGTTACTA |
OVM094 | GTTT | 5 | 163 | ATTCATGGGGACAATAAATTC | CAAGAGAATGAATGAATCAGC |
OVM099 | GA | 9 | 149 | TATGTATTGCAGAATCACAGC | TATTACCCTTTTCCATCTTCC |
OVM100 | AAG | 6 | 151 | GAACTAGATTTGCGGCATT | CCCACACCCTTATCCTTATTA |
OVM110 | AT | 8 | 154 | CTGGACGAAAACAACATATTC | GTTGGCTTTGGTACTGACATA |
OVM116 | GAT | 7 | 151 | AACTACACGCACGTAATGAAT | TGGTTTGATAAACACCTCAAG |
OVM120 | TTC | 6 | 152 | TTCAGTGTCACTTTCCTCATT | AGAAGTTGTCATGTCAAGGAA |
OVM122 | TGG | 6 | 156 | ATGAATCTTGTACGGAATCTG | GAAGAAAAAGCCATAAACACC |
OVM125 | AAATT | 5 | 151 | ATTCTTTCAACAAGCAAGTGA | CTGCAATTCCATCCTATTTTA |
OVM126 | TCC | 6 | 188 | ACTAAGAACCACCCAAAACAT | TGAGAAGATGGAGAAGATGTG |
OVM128 | TGTT | 6 | 155 | GAGAAGCATAACCAAAATCCT | TGGAAGAAAAGAAACTTCTGA |
OVM129 | TG | 8 | 133 | AATTGGATTCATGTGTTAGGA | GAAGTGGAGCCAAAACCT |
OVM130 | AG | 9 | 154 | GCAAATTATCACCATGCAC | CGTGAAGAAAATCGGTACTTA |
OVM131 | AGA | 6 | 153 | GAAATAACGCAGGCAGATAC | AATTAGAGGCTTCGACTTGTT |
OVM132 | GAC | 6 | 142 | ACGGTAATCAGTAGTGACAGC | GTGTGACAGAAAATGGGATTA |
OVM133 | TTTC | 5 | 171 | TAGCATCAAGGTTGGAAATAG | CTAGGCTACCTGAATCAAACA |