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Table 1 Primers used in this study

From: Haynaldia villosa NAM-V1 is linked with the powdery mildew resistance gene Pm21 and contributes to increasing grain protein content in wheat

Primer name

Primer sequence (5′-3′)

Product length

Annealing Temperature

Purpose

NAMORF1

F: GATGAGGTCCATGGGCAG

1528 bp

60 °C

Genomic DNA cloning

R: TCATTTGCTCAGGGATTCC

NAMORF2

F: ATGGGCAGCTCCGACTCA

1540 bp

60 °C

cDNA cloning

R: TCAGGGATTCCAGTTCACG

CauNAM-V1

F: TCCCCGGTATGCCATGTC

575 bp

58 °C

Specific molecular marker for NAM-V1

R: AAGATACCGCTAGACCGTGA

CauNAM-ABD

F: TACAAGTTCGACCCATGGGA

265 -294 bp

58 °C

Molecular marker for NAM-A1, B1, D1, B2 and D2

R: GCGCGGGCTGAAGAAGTA