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Table 2 Protein differentially expressed for Shaziling pig breeds and Yorkshire were identified by 2-DE and MALDI-TOF-MS/MS

From: Integrative analysis of transcriptomics and proteomics of skeletal muscles of the Chinese indigenous Shaziling pig compared with the Yorkshire breed

Sport no

NCBI accession number

Protein name

Mr, kDa theor

pI theor

No. of peptides identified

Mascot score

Sequence coverage

Expecte

Up-regulated in Yorkshire pigs

 1375

gi|89574051

mitochondrial ATP synthase, H+ transporting F1 complex beta subunit, partial, ATP5B

47059.6

4.99

16

1180

46 %

5.8e-11

 1929

gi|283993079

L-gulonate3-dehydrogenase

35433.2

5.79

14

417

51 %

1.2e-03

 2268

gi|545835136

PREDICTED: NADH dehydrogenase ubiquinone flavoprotein 2 isoform X1

25812.2

6.96

14

435

60 %

1.8e-039

 2247

gi|55926209

heat shock protein beta-1

22984.7

6.23

9

423

41 %

2.9e-03

 2393

gi|809283

Chain B, Structure Determination Of Aquomet Porcine Hemoglobin At 2.8 Angstrom Resolution

16082.4

6.76

12

511

86 %

4.6e-04

 2377

gi|545848507

PREDICTED: alpha-crystallin B chain isoform X2

20116.4

6.76

9

212

51 %

3.7e-01

 2497

gi|494389

Chain A, High Resolution X-Ray Structures Of Pig Metmyoglobin

16901.8

6.5

9

529

59 %

7.3e-04

 2500

gi|494389

Chain A, High Resolution X-Ray Structures Of Pig Metmyoglobin

16901.8

6.5

12

596

82 %

1.5e-05

 2575

gi|55926217

cytochrome c oxidase subunit 5B, mitochondrial precursor

14002

8.8

8

334

46 %

2.3e-02

 2594

gi|809283

Chain B, Structure Determination Of Aquomet Porcine Hemoglobin At 2.8 Angstrom Resolution

16082.4

6.76

9

400

67 %

5.8e-03

Up-regulated in Shaziling pigs

 2480

gi|117660856

MYLPF

19.0

4.8

6

110

36 %

5.8e-00

 2395

gi|117660874

MLC1f

21018.6

4.9

11

444

60 %

2.3e-40

 2654

gi|117660856

MYLPF

19066.3

4.8

9

347

49 %

1.2e-03

 2422

gi|117660890

MLC3f

16761.2

4.6

4

250

29 %

2.3e-02

 2659

gi|117660856

MYLPF

19066.3

4.8

6

144

27 %

2.3e-01

 2400

gi|545858131

keratin, type I cytoskeletal 10 isoform X2

58318.6

4.9

16

190

21 %

5.8e-01

 2482

gi|117660856

MYLPF

19066.3

4.8

16

600

86 %

5.8e-05

 2522

gi|117660874

MLC1f

21018.6

4.9

8

190

40 %

5.8e-01

 1154

gi|545845559

PREDICTED: fibrinogen beta chain isoform X2

50399.8

7.94

18

gi|545845559

43 %

4.6e-03

 2225

gi|262263205

triosephosphate isomerase 1

26878.9

6.54

14

463

74 %

2.9e-04

 2203

gi|262263205

Triosephosphate somerase 1

26878.9

6.54

17

774

77 %

2.3e-07

 1326

gi|311247991

PREDICTED: pyruvate dehydrogenase protein Xcomponent-like isoform

54036.4

8.3

12

317

28 %

1.2e-02

 1416

gi|545801458

PREDICTED: actin, alpha cardiac muscle 1 isoform X1

42334

5.2

10

313

31 %

2.9e-02

 2250

gi|262263205

Triosephosphate isomerase 1,TPI1

26878.9

6.5

17

795

82 %

1.8e-07

 2584

gi|314907119

A-FABP adipocyte fatty acid-binding protein

14780.6

6.2

8

371

56 %

4.6e-03

 1433

gi|545833443

PREDICTED: alpha-enolase isoform X1

38172.7

8.93

12

377

41 %

1.2e-03

 2221

gi|55926209

HSPB1 heat shock protein beta-1

22984.7

6.23

8

236

31 %

1.5e-01

 1805

gi|545859898

PREDICTED: beta-enolase isoform X1

48154.9

8.52

17

414

40 %

2.3e-03

 2550

gi|545805333

PREDICTED: 14 kDa phosphohistidine phosphatase isoform X2

14036.8

5.91

14

454

74 %

2.3e-04

 2217

gi|262263205

triosephosphate isomerase 1

26878.9

6.54

16

701

82 %

4.6e-06

 1711

gi|46389777

troponin T fast skeletal muscle type

30720.1

8.68

14

423

41 %

2.9e-03

 1137

gi|545845559

PREDICTED: fibrinogen beta chain isoform X2

50399.8

7.94

15

289

34 %

7.3e-02

 2215

gi|262263205

triosephosphate isomerase 1

26878.9

6.54

18

684

76 %

2.3e-06

 2223

gi|55926209

HSPB1 heat shock protein beta-1

22984.7

6.23

10

371

42 %

4.6e-03

 1833

gi|545832797

PREDICTED: troponin T, slow skeletal muscle isoformX1

32449.6

5.54

9

418

32 %

9.2e-03

 1374

gi|268607671

actin, alpha skeletal muscle

42366

5.23

11

348

35 %

9.2e-03

  1. eNumber of times we would expect to obtain an equal or higher score by chance