Fig. 4

Panel construction in Fragman package. Visual output of the overview2 function implemented in Fragman to create panels of potential alleles found in a population. The function overlaps the fluorescent intensities in all the loaded FSA files for the selected channel. Here, the fluorescent emission intensity of FAM labeled DNA fragments in 288 diploid plants from a diversity panel are plotted to show the allelic variability for this genetic marker. The function by default suggests and returns all peaks with minor allele frequency (MAF) > 0.05 along with a plot which can be used interactively with the use of the locator function to manually select alleles