Fig. 3

A 1 bp deletion in ARMC3 induces premature translation termination. a Snapshot from the Integrated Genomics Viewer (IGV, [51]) showing a homozygous 1 bp deletion on chromosome 13 at 24,301,425 bp in an animal with the tail stump sperm defect. b Genomic structure of bovine ARMC3. Bovine ARMC3 consists of 19 exons (vertical bars) and its translation starts in exon 2. The red vertical bar represents the eleventh exon where the 1 bp deletion is located. The coordinates of ten Armadillo (ARM) repeats were determined using the Simple Modular Architecture Research Tool [50]. Blue arrows represent the position of the start and stop codons. c The bovine ARMC3 protein sequence consists of 876 amino acids and it contains ten ARM repeats (green boxes). The red triangle represents the start of the shift in translation resulting from the 1 bp deletion. d Multi-species alignment of a part of the ARMC3 protein sequence. Blue colour highlights the protein sequence of the tenth ARM repeat, which is absent in the mutated (mt) bovine sequence