Table 4 Characterization of the β-LCY and ε-LCY genes: gene size (bp), number of exons and introns, the predicted regions by CODDLe program in terms of exon region and region length (bp) and the considered amplicon size for the TILLING strategy
From: DHPLC technology for high-throughput detection of mutations in a durum wheat TILLING population
Gene | Size (bp) | Number of exons | Number of introns | CODDLe prediction | TILLING approach | |
|---|---|---|---|---|---|---|
Exon region | Length (bp) | Amplicon size (bp) | ||||
β-LCY-6A | 1,703 | 1 | 0 | 1 | 400 | 880 |
β-LCY-6B | 1,455 | 1 | 0 | 1 | 400 | 875 |
ε-LCY-3A | 4,652 | 10 | 9 | 4–8 | 1,299 | 1,163 |
ε-LCY-3B | 4,435 | 10 | 9 | 4–8 | 1,299 | 789 |