Fig. 2From: DHPLC technology for high-throughput detection of mutations in a durum wheat TILLING populationOutline of SNP detection by heteroduplex analysis of PC133f-PC130r amplicons of ε-LCY-3A gene. The chromatograms correspond to elution profile of homoduplex samples (cv. Saavi DNA in brown line and cv. Casanova DNA in dark green line) and the double peak of heteroduplex mixed DNA (green line). All runs were conducted at the same melting temperature in DNA Rapid program (57.7 °C)Back to article page