|
Primer
|
Target species
|
In silico PCR positive amplification, Ampliflix
|
NCBI Primer BLAST match with non-target species
|
PCR Positive amplification
|
|---|
|
Forward
|
Reverse
|
|---|
|
TIG 490a
|
tiger
|
tiger
|
Hyena
|
lion, hyana (striped & spotted), lynx, puma
|
tiger, leopard, lion
|
|
TIG 509b
|
tiger
|
tiger
|
tiger, leopard
|
puma
|
tiger, leopard, lion
|
|
NADH4c
|
leopard
|
lion, tiger, hyena
|
lion, leopard
|
lion
|
leopard, lion, clouded leopard, tiger
|
|
TSPd
|
tiger
|
tiger
|
tiger
|
-
|
tiger
|
|
LSPe
|
leopard
|
leopard
|
leopard
|
-
|
leopard
|
-
a,b – Mukherjee & Mondol et al., 2007 [15]; c– Mondol et al., 2009 [17]; d– Bhagavatula et al.,2006 [16]; e– This study
