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Table 1 Results of various specificity tests for tiger and leopard specific primers available in literature

From: Schrodinger’s scat: a critical review of the currently available tiger (Panthera Tigris) and leopard (Panthera pardus) specific primers in India, and a novel leopard specific primer

Primer

Target species

In silico PCR positive amplification, Ampliflix

NCBI Primer BLAST match with non-target species

PCR Positive amplification

Forward

Reverse

TIG 490a

tiger

tiger

Hyena

lion, hyana (striped & spotted), lynx, puma

tiger, leopard, lion

TIG 509b

tiger

tiger

tiger, leopard

puma

tiger, leopard, lion

NADH4c

leopard

lion, tiger, hyena

lion, leopard

lion

leopard, lion, clouded leopard, tiger

TSPd

tiger

tiger

tiger

-

tiger

LSPe

leopard

leopard

leopard

-

leopard

  1. a,b – Mukherjee & Mondol et al., 2007 [15]; c– Mondol et al., 2009 [17]; d– Bhagavatula et al.,2006 [16]; e– This study