Primer | Target species | In silico PCR positive amplification, Ampliflix | NCBI Primer BLAST match with non-target species | PCR Positive amplification | |
---|---|---|---|---|---|
Forward | Reverse | ||||
TIG 490a | tiger | tiger | Hyena | lion, hyana (striped & spotted), lynx, puma | tiger, leopard, lion |
TIG 509b | tiger | tiger | tiger, leopard | puma | tiger, leopard, lion |
NADH4c | leopard | lion, tiger, hyena | lion, leopard | lion | leopard, lion, clouded leopard, tiger |
TSPd | tiger | tiger | tiger | - | tiger |
LSPe | leopard | leopard | leopard | - | leopard |