Fig. 3From: Genome-wide identification of copy number variation using high-density single-nucleotide polymorphism array in Japanese Black cattleQuantitative PCR (qPCR) validation of CNVR_285 (a), CNVR_437 (b), and CNVR_631 (c). The left-most bar in each panel represents a calibrator animal (JB_001). The calibrator animal is assumed to contain two copies of the DNA segment detected from the PennCNV analysis. The Basic transcription factor 3 gene (BTF3), which served as an internal qPCR standard for both copies at a locus (2n), was co-amplified with the primers. The x-axis represents the animals. The brackets represent CNVR-detected animals using the Illumina BovineHD BeadChip Array. Error bars represent ± Standard Error of Mean (SEM) obtained from triplicate experimentsBack to article page