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Fig. 3 | BMC Genetics

Fig. 3

From: Genome-wide identification of copy number variation using high-density single-nucleotide polymorphism array in Japanese Black cattle

Fig. 3

Quantitative PCR (qPCR) validation of CNVR_285 (a), CNVR_437 (b), and CNVR_631 (c). The left-most bar in each panel represents a calibrator animal (JB_001). The calibrator animal is assumed to contain two copies of the DNA segment detected from the PennCNV analysis. The Basic transcription factor 3 gene (BTF3), which served as an internal qPCR standard for both copies at a locus (2n), was co-amplified with the primers. The x-axis represents the animals. The brackets represent CNVR-detected animals using the Illumina BovineHD BeadChip Array. Error bars represent ± Standard Error of Mean (SEM) obtained from triplicate experiments

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