Skip to main content

Table 1 Pairs of primers used for HRM screening, sequencing and genotyping ABCC1

From: Genetic variation of the ABC transporter gene ABCC1 (Multidrug resistance protein 1 – MRP1) in the Polish population

 

Exon

Number of scanned bp upstream from the exon (without primer)

Forward primer (5’ → 3’)

Reverse primer (5’ → 3’)

Number of scanned bp downstream from the exon (without primer)

Size (bp) including primers

Scanning

1

104*

CAGCGCTAGCGCCAGCAG

CCCGGTGCTTTCCCTCCC

49

234

2

31

GTCCTCTGGGGTGTGTCCT

TCTGAATGTAGCCTCGGTCA

 

201

  

GCTTTCAGAACACGGTCCTC

TTATCACCAACCACCACTCC

52

203

3

20

GGGCGGTCTGTTGTAGGATA

CCACTGAGCCCAGCAGAT

31

215

4

129

AAGCTGAGGCAGGAGAATCA

AAGGTAGCAAGCAGCTGAGG

 

163

  

AGCCTGGGTGACAAGAGTGA

TGGATCTCAGGATGGCTAGG

 

189

  

ATGCTCACTTTCTGGCTGGT

GGCAACGCATGACTTCTACA

72

170

5

27

CAGCCCCAGAATGTGATCTT

CACACACACGCACACACACT

19

212

6

20

TTTCCCTCTTCCTCCCAAAC

AGCTGAGCATGTTCATTCGTT

59

182

7

33

CCCTCCTCCTGTCATTGACTC

GAGTGGAAAGGAGGTGACCA

 

240

  

GGAACAAGTCGTGCCTGTTT

ATCTTGCCCAGAACCACAAA

104

184

8

74

TTCCCTGAAGGGTGACATTC

AGGGGTTCCACTCCTTCTGT

 

219

  

GGAGGCTTTGATCGTCAAGT

AAAGCCAAGGAGGGAAAATG

29

199

9

14

AGTCATTCCAGGCCCTCTCT

TGACGAAGCAGATGTGGAAG

 

165

  

TACACCGTGCTGCTGTTTGT

CAGGAGGGGATGTGGAAGT

33

175

10

27

TCTTCAGCTGCCACACTCAC

GCCACAGGAGGTAGAGAGCA

 

207

  

TCTGTGGACGCTCAGAGGTT

CTGCCCACACGTAGAAGTCC

38

157

11

21

TCTTCTGTCTGGTGAGTGATGAA

TGGACTAAAATCCTCATGGAGAG

49

209

12

41

GTTGAGTGATGGGCTGATCC

GGCAGACTTCTTCAGCACCT

 

217

  

GCATTCAAGGACAAGGTGCT

CCTGGGCAACATAGTGACCT

75

206

13

62

GTCTCCAGGGCCTGTCACT

ACCGGAGGATGTTGAACAAG

 

184

  

CCAGACAGCCTTCGTGTCTT

GCTTTCGTGGCCTAGAACC

42

149

14

49

TTAACCTTGGTTGGTTTTGC

CAAGTAGGGAGGACCCAGTG

51

228

15

41

GTTCTGTGCATGTGGAGTCG

GGCTCTCTCCACACCATCAC

22

179

16

73

TTCTCTACTTGGGGTAAATTGAGG

CTGAGAGCAGGGACGACTTT

 

177

  

ATCCCCGAAGGTGCTTTG

GCTTTTCCTCAGACCACCAG

37

171

17

12

CTGTCTCACCTCGTTCTCCA

CTGACCTTCTCGCCAATCTC

 

214

  

CTCCCAGACCTGGAAATCCT

CAGCCCACTGAGATTGTGAG

60

134

18

20

ATTTCCCAGGAAACCCACTC

GGCCAATCACATTTTCAAAGA

 

185

  

CTTCGATGATCCCCTCTCAG

CCTAAAGGGGACACGTTCTG

55

166

19

26

CTCACACATGTGCACTCACG

TCTGTGCTGGCATAGGTACG

 

204

  

GCCAAGCTAGGCAGTCTCAC

GGCAAGTAGCTCATGCTGTG

 

99

  

TCTGAGATGGGCTCCTACCA

CAACCTCAAAGAGGCCAATG

56

182

20

48

TTGTTGCCCTTGGTTTTAGC

CTCCTCTTGTCCTCCCACTG

44

223

21

20

GCATCTGTACGGTTGACACC

TGGGCGCTCTATAAACTCCA

32

228

22

25

GGTGCGTGCATGTGCTAAG

ATGGGGTCATCAGTCCAGAG

 

184

  

GGCTTCCAACTATTGGCTCA

CAAAACGCTGAGGACTCTAAGG

72

204

23

21

CCCTCTCTGCATTGTGGAGT

GTCCACTGTGTCCAGCTCCT

 

214

  

ATGAGCTTCTTTGAGCGGAC

GGCTTGTCCCGAACACTAAG

14

244

24

22

GAGGGAACCTTCATCAACTCC

TCTGGTTCTCGTCCACCTTC

 

212

  

CCCATTTCAACGAGACCTTG

GGAAAAGATCGAGGCAAAGA

55

208

25

26

AGAGCTGACTCCATGCCTGT

GGCTCCCCCATTAACAGACT

32

225

26

24

CGCCCGCTTACTCTAGAAAT

CCAAAGACCAAGAGGTCCAG

18

184

27

45

GGGGAGTCACAGCTTTACCA

GGGAATGGGTGAGGGAAT

18

248

28

6

ACACCTGGGCCCTTCTGTC

TGATCTCTCCTTCGGCAGAC

 

113

  

CTGGGCTTATTTCGGATCAA

CAGTGCAATCATAGGGCTTG

40

174

29

55

AGTCCTTAGGTCGCCTCCAT

CCTTCTGCACATTCATGGTC

 

227

  

CCCACCTGAAGGACTTCGT

CAAACACCCCTACCGAGATG

47

143

30

74

GTGTCTCCTTTCGCTTCTCC

ATGGTGGACTGGATGAGGTC

 

217

  

CCTGCTGAGGAAGACGAAGA

AGGCAAACTCCCAAAGCCTA

25

204

31

15

CCCTTCCCCTCATGTCTGTA

ATAGGCCCTGCAGTTCTGAC

21**

185

Sequencing

1

 

-

-

 

-

2

 

TCTCGAACTCCCAGCCTAAA

TAAGCGGCAGAGCAAAGATT

 

749

 

3

 

GAGCATGGTGACCAGACAAA

CTCCTGACCTCAGGTGATCC

 

638

4

 

GTGGTGAAACCCCGTCTTTA

CCTTGGAGCAACACAGACAA

 

604

5

 

CCCGAGTAGCTGGGATTACA

ACCATGCCAAGTGAGAAACC

 

606

6

 

GGGTTGTTGTGGGGATAGAA

AAGCAAAAGCAGTCCAGCAT

 

622

7

 

CTGCATGACCCAACAGAATG

CAAACTCCCGACCAAGTGAT

 

651

8

 

CTTTGACTCTGCCTTCCCTG

TAGGGGAGCCTAGTGGGTTT

 

691

9

 

AGCAATTAGTGCACAACCCC

AAGACATCTACACGCCCACC

 

613

10

 

ACAGCCTGGAAGCGTAGAAA

AACTGCCAGGGAGCTCTACA

 

664

11

 

GAGCGTGGACCTGCTTATTC

CACCGGCCTACAAGGTCTAA

 

608

12

 

GATGTTGAGTGATGGGCTGA

CCTGGGCAACATAGTGACCT

 

363

13

 

ATACTGCCCCAGGTTTTTCC

AAAAAGGATAGGAATGGCGG

 

611

14

 

CTGGGGAAACCCTTGAAAGT

CCAAGGGAAAGAAATGCAAG

 

263

15

 

CCCAAGATGATCGTCCAGAT

TACGCAAGCCAAGAATCTCC

 

632

16

 

TCTCCTTTCAGACCTTGCGT

CCTGCCTTCTAGGACAGCAC

 

676

17

 

ACTCTTTGGAAGCAGGGGTT

AGTGAGACCTGAGCCACACC

 

693

18

 

CTCAAGCCATCTTCCCACTC

GAAGCCAGCCCTGTGACTTA

 

717

19

 

CATGTCCCACCTTCAGACCT

CCAGCTTAACTCCGTGCTTC

 

748

20

 

CTGCACAGTTGCAAAGCACT

GCCAAGAGACCTGAGCAAAG

 

620

21

 

CCGTCTCTTATGCCATGGTT

GGTTCAAATCCCAGTTCTGC

 

461

22

 

GCATGTGCTAAGCTGCCTTAT

TGTAAAATGGGCACACTGGA

 

485

23

 

CTCAAGTGATCCACCCACCT

TGCTTCAAAAGCACCACACT

 

617

24

 

GAGGTTAGCACTTTGCAGCC

ATGATTCATCTGTCCTCCGC

 

791

25

 

AAGGCAAGCTTCAGATTCCA

AAAGCATTCCCCTCGTACCT

 

674

26

 

AAATGCCACGTGACTCTTCC

CCTGGTGAGGTATCCAGCTC

 

242

27

 

AGGGGACAGAGGGACACAG

AAATCTGTGGGGCTCATTTG

 

618

28

 

TCATGATGGGAAACTCACCA

GAACGATGAAGTAGGGCCAA

 

669

29

 

AGACAGGGTGTTGCCATGTT

TCAACTGAATGGAGCTGGTG

 

689

30

 

GGTGTGAGCTGCTGTTTTGA

AAGAGAGGATCCACCCACTG

 

556

31

 

CCATGATTGATGTGGGGTAG

AAGCACCAGGAAACCACTTG

 

640

Genotyping

SNP

Forward primer (5’ → 3’)

Reverse primer (5’ → 3’)

 

Size (bp) including primers

 

c.128G > C

 

TCAGAACACGGTCCTCG

ACAGGCCCAGAGGTAAAA

 

49

c.616-7942A > G

 

AGCAACAGGGCAAACAAATC

GACGGAGCCTAAATGTCCAG

 

76

c.816G > A

 

CTTGTGTTCCAGGCAGC

GCAGGATCCTTGGAGGAGTA

 

51

c.825 T > C

c.1057G > A

 

GCTCCTTTGCAGGTTGCTCATC

GGGGCCTTCGTGTCATTCA

 

48

c.1062 T > C

 

GGTTGCTCATCAAGTTCGTGA

GTCTGGGGCCTTCGTGTC

 

41

c.1218 + 8A > G

 

CATGAGGATCAAGACCGCTG

GTGGAAGTCGGGCCACAT

 

84

c.1299G > T

 

CCAGAAAATCCTCCACGGT

GTACGTGGCCAAGTCCATGA

 

80

c.1684 T > C

 

GTTCTGCTTGCAGGAGGCC

TTCAGGGGAAACCGGAGGAT

 

125

c.1704C > T

c.2012G > T

 

TCACCTTCTCCATCCCCG

CACTTTGTCCATCTCAGCCA

 

104

c.2168G > A

 

CAGGCCTGGATTCAGAATGA

ATGGTTCCTCCAGCTGACAT

 

67

c.2581G > A

 

CAGGAGCTGCTGGCTCG

TCTGTGCTGGCATAGGTACG

 

62

c.2965G > A

 

TCCTTTTCATGTGTAACCATGTG

CAATAGTTGGAAGCCAGCG

 

46

c.3140G > C

 

CTACTCCATGGCCGTGTCC

AGGATGCTGTGCAGCAGGT

 

75

c.3436G > A

 

GAGGTTCTACGTGGCTTCC

GAATAGACCGGGGAGCG

 

69

c.4002G > A

 

GACAAGTCCGGATGCCAG

CGAAATAAGCCCAGGGTC

 

112

c.4009A > G

 

AGCTGGGAAGTCGTCCCT

TGATCTCTCCTTCGGCAG

 

65

  1. 104* - upstream from the START codon. 21** - downstream from the STOP codon. Bold variants means these ones which genetic variation was detected during genotyping method