Diet-induced variability of the resistin gene (Retn) transcript level and methylation profile in rats

BMC Genomic Data

Table 1 PCR primer sequences, amplicon length and annealing temperature used in Retn gene analysis

Type of analysis	Gene	Primer sequence	Amplicon length	Anneal.temp.
Transcript level analysis	Retn	F: 5′ CCACGTACTTAACAGGATG 3′	195 bp	62 °C
	Retn	R: 5′ GAGGAGACTGACCAGCAAT 3′	195 bp	62 °C
	Hprt	F: 5′ CAGTCAACGGGGGACATAAAAG 3′	146 bp	62 °C
	Hprt	R: 5′ ATTTTGGGGCTGTACTGCTTGA 3′	146 bp	62 °C
	Tbp	F: 5′ ATCCTTCACCAATGACTCCTATG 3′	190 bp	62 °C
	Tbp	R: 5′ ATGATGACTGCAGCAAACC 3′	190 bp	62 °C
Methylation analysis (converted sequence)	Retn	F: 5′ GTGGAAAGGAGGAATGTATTATTTG3′	249 bp	56 °C
Methylation analysis (converted sequence)	Retn	R: 5′ACAAATAAAAAAACTTTAATATTTATTCA3′	249 bp	56 °C

Reference sequences for the Retn - GenBank: NC_005111.3; the Hprt - GenBank: S79292 and the Tbp – GenBank:NM_001004198

ISSN: 2730-6844