Skip to main content

Table 2 Primer pair sequences and PCR conditions of the studied genetic marker

From: Polymorphism profiling of nine high altitude relevant candidate gene loci in acclimatized sojourners and adapted natives

Gene variant

NCBI rsID

Primer sequence

PCR cycle

PCR cycling conditions

RE

RE Condition

Agarose (%)

Allele sizes (bp)

EDN1 9456G > A

rs2071942

F: 5’ CAAACCGATGTCCTCTGTA 3’

35

I 95 °C 5’, D 95 °C 1’, A 52 °C 1’, E 72 °C 2’, FE 72 °C 7’

Taq αI

65 °C, 2 h

1.8

G: 150,208

R: 5’ ACCAAACACATTTCCCTATT 3’

A: 358

EDN1 3A/4A (−134 del)

rs1800997

F: 5’ GCTGCCTTTTCTCCCCGTTTAA 3’

30

I 95 °C 2’, D 95 °C 30”, A 57.2 °C 30’, E 72 °C 20”, FE 72 °C 5’

Bs1I

5 °C O/N

3

3A:221

R: 5’ CAAGCCACAAACAGCAGAGA 3’

4A:197, 24

SCNN1B Thr594Met (1781C > T)

rs1799979

F: 5’ ACCGTGGCCGAGCTGGTGGAG 3’

35

I 94 °C 5’, D 94 °C 30’, A 67.5 °C 1.5’, E 72 °C 30”, FE 72 °C 5’

NlaIII

37 °C O/N

1.5

T: 226

R: 5’ CAGTCTTGGCTGCTCAGTGAG 3’

C: 117,109

ADRB2 Arg16Gly (46A > G)

rs1042713

F: 5’-CTTCTTGCTGGCACGCAAT-3’

30

I 94 °C 10’, D 94 °C 1’, A 57 °C 1’,72 °C 1’, FE 72 °C 5’

BsrDI

65 °C 21/2 hr

3

A:131,56,14

R: 5’-CCAGTGAAGTGATGAAGTAGTTGG-3

G: 14, 23, 56, 108

ADBR2 Gln27Glu (79G > C)

rs1042714

F: 5’-GGCCCATGACCAGATCAGCA-3’

30

I 94 °C 10’, D 95 °C 1’, A 63 °C 1’, E 72 °C 1’, FE 72 °C 5’

Fnu4HI

37 °C, 3 h

3

C: 229, 97, 27

R: 5’-GAATGAGGCTTCCAGGCGTC-3’

G: 174, 97, 55, 27

ADBR3 Try64Arg (5387 T > C)

rs4994

F: 5’-CAATACCGCCAACACCAGTGG-3’

30

I 95 °C 10’, D 95 °C 30”, A 60 °C 30’, E 72 °C 30”, FE 72 °C 5’

MspI

37 °C, O/N

3

T: 99, 54

R: 5’-GGTCATGGTCTGGAGTCTCG-3’

C: 70, 54, 29

VEGF 963C > T

rs3025039

F: 5’-AGGAAGAGGAGACTCTGCGCAGAG

30

I 94 °C 2’, D 94 °C 30”, A 65 °C 40’, E 72 °C 40’, FE 72 °C 5’

NlaIII

37 °C, 3 h

3

C: 208

CAGGAAGAGGAGACTCTGCGCAGAGC-3’

T: 122, 86

R: 5’-TAAATGTATGTATGTGGGTGGGTGTGTCTACAGGG-3’

 

eNOS Glu298Asp (894 T > G)

rs1799983

F: 5’-CATGAGGCTCAGCCCCAGAAC-3’

30

I 94 °C 5’, D 94 °C 30”, A 63 °C 20’, E 72 °C 30’, FE 72 °C 10’

MboI

37 °C O/N

2.5

G: 206

 

R: 5’-AGTCAATCCCTTTGGTGCTCAC-3’

T: 119, 87

TH Va181Met (7085G > A)

rs6356

F:5’-GGCAGAGCCTCATCGAGGAC-3’

40

I 95 °C 2’, D 94 °C 1’, A 63 °C 45’, 72 °C 2.5’, FE 72 °C 10’

NlaIII

37 °C O/N

2.5

G: 197

R: 5’-AAACACCTTCACAGCTCGGGAC-3’

A: 131, 66