Gene variant | NCBI rsID | Primer sequence | PCR cycle | PCR cycling conditions | RE | RE Condition | Agarose (%) | Allele sizes (bp) |
---|---|---|---|---|---|---|---|---|
EDN1 9456G > A | rs2071942 | F: 5’ CAAACCGATGTCCTCTGTA 3’ | 35 | I 95 °C 5’, D 95 °C 1’, A 52 °C 1’, E 72 °C 2’, FE 72 °C 7’ | Taq αI | 65 °C, 2 h | 1.8 | G: 150,208 |
R: 5’ ACCAAACACATTTCCCTATT 3’ | A: 358 | |||||||
EDN1 3A/4A (−134 del) | rs1800997 | F: 5’ GCTGCCTTTTCTCCCCGTTTAA 3’ | 30 | I 95 °C 2’, D 95 °C 30”, A 57.2 °C 30’, E 72 °C 20”, FE 72 °C 5’ | Bs1I | 5 °C O/N | 3 | 3A:221 |
R: 5’ CAAGCCACAAACAGCAGAGA 3’ | 4A:197, 24 | |||||||
SCNN1B Thr594Met (1781C > T) | rs1799979 | F: 5’ ACCGTGGCCGAGCTGGTGGAG 3’ | 35 | I 94 °C 5’, D 94 °C 30’, A 67.5 °C 1.5’, E 72 °C 30”, FE 72 °C 5’ | NlaIII | 37 °C O/N | 1.5 | T: 226 |
R: 5’ CAGTCTTGGCTGCTCAGTGAG 3’ | C: 117,109 | |||||||
ADRB2 Arg16Gly (46A > G) | rs1042713 | F: 5’-CTTCTTGCTGGCACGCAAT-3’ | 30 | I 94 °C 10’, D 94 °C 1’, A 57 °C 1’,72 °C 1’, FE 72 °C 5’ | BsrDI | 65 °C 21/2 hr | 3 | A:131,56,14 |
R: 5’-CCAGTGAAGTGATGAAGTAGTTGG-3 | G: 14, 23, 56, 108 | |||||||
ADBR2 Gln27Glu (79G > C) | rs1042714 | F: 5’-GGCCCATGACCAGATCAGCA-3’ | 30 | I 94 °C 10’, D 95 °C 1’, A 63 °C 1’, E 72 °C 1’, FE 72 °C 5’ | Fnu4HI | 37 °C, 3 h | 3 | C: 229, 97, 27 |
R: 5’-GAATGAGGCTTCCAGGCGTC-3’ | G: 174, 97, 55, 27 | |||||||
ADBR3 Try64Arg (5387 T > C) | rs4994 | F: 5’-CAATACCGCCAACACCAGTGG-3’ | 30 | I 95 °C 10’, D 95 °C 30”, A 60 °C 30’, E 72 °C 30”, FE 72 °C 5’ | MspI | 37 °C, O/N | 3 | T: 99, 54 |
R: 5’-GGTCATGGTCTGGAGTCTCG-3’ | C: 70, 54, 29 | |||||||
VEGF 963C > T | rs3025039 | F: 5’-AGGAAGAGGAGACTCTGCGCAGAG | 30 | I 94 °C 2’, D 94 °C 30”, A 65 °C 40’, E 72 °C 40’, FE 72 °C 5’ | NlaIII | 37 °C, 3 h | 3 | C: 208 |
CAGGAAGAGGAGACTCTGCGCAGAGC-3’ | T: 122, 86 | |||||||
R: 5’-TAAATGTATGTATGTGGGTGGGTGTGTCTACAGGG-3’ | ||||||||
eNOS Glu298Asp (894 T > G) | rs1799983 | F: 5’-CATGAGGCTCAGCCCCAGAAC-3’ | 30 | I 94 °C 5’, D 94 °C 30”, A 63 °C 20’, E 72 °C 30’, FE 72 °C 10’ | MboI | 37 °C O/N | 2.5 | G: 206 |
R: 5’-AGTCAATCCCTTTGGTGCTCAC-3’ | T: 119, 87 | |||||||
TH Va181Met (7085G > A) | rs6356 | F:5’-GGCAGAGCCTCATCGAGGAC-3’ | 40 | I 95 °C 2’, D 94 °C 1’, A 63 °C 45’, 72 °C 2.5’, FE 72 °C 10’ | NlaIII | 37 °C O/N | 2.5 | G: 197 |
R: 5’-AAACACCTTCACAGCTCGGGAC-3’ | A: 131, 66 |