Table 1 Primer-pairs, repeat motif and allele size range of newly developed EST-SSR loci
Locus name | SSANb | forward primer (5′- 3′) | reverse primer (3′- 5′) | Expected sizes | Repeat motif | Allele size range |
|---|---|---|---|---|---|---|
PS01 | cl191ct198cn251e00 | TTCGTTTTGGTTACGATCGAG | AGGTGTGGTTCAGAGGCTGT | 247 | (ATT)7 | 244-253 |
PS04 | cl41ct42cn53e00 | CAGCACTATCAATCACCACACTG | GCGTTGTTCCTGTTTCACCT | 292 | (AAT)14 | 265-298 |
PS05a | PSS08K23u | TGCATGCAGGACACTTGTAG | AAAAGTGCGTGGACCTGAAC | 291 | (AGAGTT)5 | 261-297 |
PS08 | PSC22C12r | TCACAGGAGGAAAAGGATGG | GGAGGGGGTTTGGTAAAAAG | 189 | (CCT)6 | 168-198 |
PS09a | PSC23H12u | TCAAGGGGAAACTGGAAAAA | TCATGATGTGGAGGCCAAG | 220 | (GGT)6 | 223-229 |
PS10 | PSC24E20u | TCCATTCTCCAACAACACTAACA | TGGCTGAACTCACCAAACAA | 396 | (ATC)7 | 405-450 |
PS11 | PSC25H08u | TGGGAAACTTGAGCCTACGA | TGAAACAGCCTCTTCTTCAATTA | 299 | (GGCGGT)4 | 299-311 |
PS13 | PSC28N15u | TCCTACCTACCTACCCTTTCCA | TGTGGACCCCACCATAAGAC | 234 | (ATC)7 | 225-261 |
PS15 | PSC30D03r | AGAATGGCGAGACAACGAAG | AACAACAGGTGCCAAGGAAG | 204 | (GGT)7 | 210-213 |
PS16 | PSC32L05u | TTTCTGCTTCTATTGCATGTTACC | TCCATTTGCTACACCAATATTTTCT | 250 | (ATT)7 | 256-286 |
PS18 | PSC33A19r | AGGATGGCAAGACAGAGAAGA | TGGAGGAATGGGAGGTAGTG | 156 | (CCT)6 | 156-165 |
PS20a | PSC35B08u | GGCTCCAACATGAAACAACA | TGGCTGATCCTGTCAACAAC | 217 | (AT)6 | 211-225 |
PS21 | PSC35D07u | AAGTGTACAAAATTACAATGGCACA | GTGGACCTCGTCGTTCAAGT | 393 | (AT)19 | 361-405 |
PS34 | PSS19H07u | CCGAAACAAGTTCGACAAAAA | TGTTGATGAAGCTGCCAATG | 218 | (CTT)6 | 221-224 |
PS36 | PSS21P12u | ATGGCCCAATATTCAAACGA | GCTCAAGCTGCAATAACAACC | 248 | (GTT)6 | 209-218 |