Fig. 6
Northern blot analysis of two T. cancriformis nuclear tRFs. Expression of (a) nuclear tRFGly(GCC) and (b) nuclear tRFLys(CUU) was detected in the adult stage of T. cancriformis with a northern blotting analysis using probes specific to the most-enriched tRF for each anticodon. Total RNA (1 or 4 μg) isolated from the adult stage was used. Washing treatments were performed at either 45 or 55 °C
