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Fig. 3 | BMC Genetics

Fig. 3

From: Analysis of an independent tumor suppressor locus telomeric to Tp53 suggested Inpp5k and Myo1c as novel tumor suppressor gene candidates in this region

Fig. 3

a and b Analysis of Myo1c and Hic1 protein expression in five EC and three NME samples. The results shown are representative of three independent experiments. Myo1c protein displayed down regulated in four out of five ECs tested, whereas Hic1 protein was not down regulated in the EC samples, even in those that in qPCR experiment showed a strong lowered expression of Myo1c transcript. The analysis additionally showed a very good correlation between expression of Myo1c at RNA and protein levels in the tumors tested, whereas such correlation was not detected for Hic1. EC: enodemetrail carcinoma; NME: non-malignant endometrium. c Methylation status of CpG islands in promoter regions of Hic1 (26 CpG sites) and Myo1c in a panel of 14 ECs, three parental strains (BDII, BN and SPRD) and one positive control (BDII +) samples. Methylation frequencies are color coded (black, ranges of gray and white) in five white/gray/black grades corresponding to percentages of CpG sites that were found methylated in each sample. For comparison, qPCR results for the Hic1 gene is presented as fold changes (FC) for each tumor, where negative and positive fold change values represent reduced and increased expressions, respectively. As shown, a correlation between methylation score at the Hic1 promoter and expression of this gene in tumors is lacking. No methylation was identified in the promoter region of Myo1c

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