Fig. 3
From: High resolution physical mapping of single gene fragments on pachytene chromosome 4 and 7 of Rosa
In situ physical mapping of genes on pachytene chromosomes of Rosa wichurana. FISH with Dig-labeled 45S rDNA (pTA71 plasmid) (a); Tyramide-FISH with MLO3 and P5CS genes, both labeled with biotin (b) and with AAA-2 gene, labeled with biotin (c); Tyramide-FISH with MGM gene labeled with biotin (d) and MDAR gene labeled with biotin (e); Sequential multicolor Tyramide-FISH with digoxigenin labeled MLO2 gene and biotin labeled PAL and MGM genes (f). Centromere of the chromosome that contains the physically mapped gene(s) is indicated by an arrowhead. Bar = 10 μm