Fig. 6From: Quantitative high resolution melting: two methods to determine SNP allele frequencies from pooled samplesDNA titrations using the curves method. a HRM normalized curves produced from sample batches that were mixed at gradient allelic ratios. b A linear regression showing a relationship between estimated and expected low-melting allele frequencies of the C70S236 locus. Pearson’s r = 1. Slope=0.982Back to article page