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Fig. 4 | BMC Genetics

Fig. 4

From: Quantitative high resolution melting: two methods to determine SNP allele frequencies from pooled samples

Fig. 4

Analysis of 384 loci in six individuals (Peaks method). Six adult corals were HRM-genotyped individually for 384 SNPs, then qHRM-genotyped as a pool in triplicate. The expected allele frequency was calculated by summing the genotypes for each individual (12 alleles possible), and the qHRM-estimated frequencies were calculated as described in the Materials and Methods section. The Pearson correlation between the expected and the qHRM-estimated values is 0.89. The slope of the linear regression is 0.94

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