Fig. 3From: Quantitative high resolution melting: two methods to determine SNP allele frequencies from pooled samplesDNA titrations using the peaks method. a) Probe melting peaks for different allele titrations demonstrating clear resolution among allele frequencies (15 %, 25 %, 50 %). b) Two homozygotes were mixed in varying proportions to test qHRM using SNP C45133S676 (Pearson’s r = 0.97, regression slope = 0.82) and SNP C22162S248 (Pearson’s r = 0.99, regression slope = 0.80). The qHRM estimates were tightly correlated to expected frequencies; however, qHRM appears to underestimate the true allele frequency for the low-melting allele for both SNPs considered.Back to article page