Table 1 Characteristics of 21 microsatellite loci used in present study
Primers | Primer sequences (5′-3′) | Forward label | Set | Annealing temperature | Product size (bp) | Total number of alleles |
|---|---|---|---|---|---|---|
BM1824 | F-gagcaaggtgtttttccaatc | VIC | 4 | 58 °C | 176-196 | 11 |
| Â | R-cattctccaactgcttccttg | Â | Â | Â | Â | Â |
CSSM08 | F-cttggtgttactagccctggg | VIC | 3 | 55 °C | 182-200 | 8 |
| Â | R-gatatatttgccagagattctgca | Â | Â | Â | Â | Â |
CSSM33 | F-cactgtgaatgcatgtgtgtgagc | NED | 5 | 58 °C | 144-188 | 21 |
| Â | R-cccatgataagagtgcagatgact | Â | Â | Â | Â | Â |
CSSM66 | F-acacaaatcctttctgccagctga | FAM | 4 | 60 °C | 167-207 | 19 |
| Â | R-aatttaatgcactgaggagcttgg | Â | Â | Â | Â | Â |
ETH10 | F-gttcaggactggccctgctaaca | NED | 1 | 58 °C | 185-221 | 14 |
| Â | R-cctccagcccactttctcttctc | Â | Â | Â | Â | Â |
ETH225 | F-gaacctgcctctcctgcattgg | VIC | 4 | 64 °C | 134-162 | 13 |
| Â | R-actctgcctgtggccaagtagg | Â | Â | Â | Â | Â |
ETH3 | F-gatcaccttgccactatttcct | NED | 4 | 57 °C | 90-124 | 16 |
| Â | R-acatgacagccagctgctact | Â | Â | Â | Â | Â |
HEL09 | F-cccattcagtcttcagaggt | FAM | 5 | 59 °C | 140-182 | 17 |
| Â | R-cacatccatgttctcaccac | Â | Â | Â | Â | Â |
HEL5 | F-gcaggatcacttgttaggga | VIC | 3 | 55 °C | 137-195 | 25 |
| Â | R-agacgttagtgtacattaac | Â | Â | Â | Â | Â |
ILSTS06 | F-tgtctgtatttctgctgtgg | FAM | 5 | 58 °C | 275-303 | 14 |
| Â | R-acacggaagcgatctaaacg | Â | Â | Â | Â | Â |
ILSTS11 | F-gcttgctacatggaaagtgc | NED | 1 | 58 °C | 249-273 | 10 |
| Â | R-ctaaaatgcagagccctacc | Â | Â | Â | Â | Â |
ILSTS34 | F-aagggtctaagtccactggc | VIC | 5 | 59 °C | 138-212 | 37 |
| Â | R-gacctggtttagcagagagc | Â | Â | Â | Â | Â |
ILSTS33 | F-tattagagtggctcagtgcc | PET | 3 | 55 °C | 131-163 | 16 |
| Â | R-atgcagacagttttagaggg | Â | Â | Â | Â | Â |
INRA05 | F-caatctgcatgaagtataaatat | FAM | 2 | 54 °C | 130-148 | 9 |
| Â | R-cttcaggcataccctacacc | Â | Â | Â | Â | Â |
INRA35 | F-atcctttgcagcctccacattg | FAM | 3 | 54 °C | 80-142 | 24 |
| Â | R-ttgtgctttatgacactatccg | Â | Â | Â | Â | Â |
INRA63 | F-atttgcacaagctaaatctaacc | PET | 2 | 54 °C | 162-190 | 14 |
| Â | R-aaaccacagaaatgcttggaag | Â | Â | Â | Â | Â |
MM12 | F-caagacaggtgtttcaatct | PET | 4 | 52 °C | 88-134 | 21 |
| Â | R-atcgactctggggatgatgt | Â | Â | Â | Â | Â |
MM8 | F-cccaaggacagaaaagact | NED | 2 | 55 °C | 114-144 | 12 |
| Â | R-ctcaagataagaccacacc | Â | Â | Â | Â | Â |
TGLA122 | F-ccctcctccaggtaaatcagc | VIC | 1 | 58 °C | 133-179 | 20 |
| Â | R-aatcacatggcaaataagtacatac | Â | Â | Â | Â | Â |
TGLA227 | F-cgaattccaaatctgttaatttgct | PET | 2 | 55 °C | 67-119 | 17 |
| Â | R-acagacagaaactcaatgaaagca | Â | Â | Â | Â | Â |
TGLA53 | F-gctttcagaaatagtttgcattca | FAM | 1 | 58 °C | 142-184 | 21 |
| Â | R-atcttcacatgatattacagcaga | Â | Â | Â | Â | Â |