Targeted Recombinant Progeny: a design for ultra-high resolution mapping of Quantitative Trait Loci in crosses between inbred or pure lines

Table 4 Population size for TRP-F2 design, polygenic effect present ¹

C	s	c	N _TG3	N _G2	N _TRP	N _F2	N _TRP /N _F2
0.20	2	0.100	3796	211	4007	3750	1.068
0.20	4	0.050	7591	400	7991	7500	1.065
0.20	10	0.020	18978	968	19946	18750	1.064
0.10	2	0.050	3796	400	4196	7500	0.559
0.10	10	0.010	18978	1917	20895	37500	0.557
0.05	2	0.025	3796	779	4575	15000	0.305
0.05	5	0.010	9489	1917	11406	37500	0.304
0.05	10	0.005	18978	3814	22793	75000	0.304
0.02	2	0.010	3796	1917	5713	37500	0.152
0.02	4	0.005	7591	3814	11406	75000	0.152
0.02	10	0.002	18978	9508	28486	187500	0.152

¹Abbreviations: C, the original QTL in Morgans; s, the reduction factor; c, target QTL in Morgans; N_TG3, total size of G₃ population; N_G2, total size of G₂ population. N_TRP, total number required across G₂ and G₃ populations; N_F2, population size for equivalent QTL width using an F₂ design. Assumptions: polygenic effect present; standardized allele substitution effect, d = 0.2; reproductive potential of the G₂ generation, n = 50; and confidence level set at (1-α) = 95%

ISSN: 2730-6844