Exome sequencing of a family with lone, autosomal dominant atrial flutter identifies a rare variation in ABCB4 significantly enriched in cases

Table 3 Primer Sequences Used in the Validation Analysis

Gene	Forward Primer ( 5 ′- 3 ′)	Reverse Primer ( 5 ′- 3 ′)	Tm (° C )
SPAM1	CAGAAATCTTGCTTGCTCCTAG	TTCAAGTGTCGGTTTTCCAC	58
IFITM10	CAGCACCACGGACGGC	GGCAGGGGGCTTGGAC	64
CACNA2D1	CTGTGTTAGGTAACGCGGAT	CTGAAAAACACCCACAACTG	57
ABCB4	CTGCTAGACATGGCTGCCAG	TTCATTTTGGACTTTGGCAGC	62
FBXO22	CCTCTGGATATTGATGCCTC	CTTTCTAAATGCATCAGCCTC	57
CEP164	TCTTTGACTCCTGATTGTGGG	CTCTTGCTTGGATTCCAGCAG	61
TRIM54	TTCATGCTTAAGGTCCACCTC	ACAGTCCTTGTGGGCACCGAAG	63

ISSN: 2730-6844