The effects of amino acids on heat shock, mitochondrial unfolded protein response, and ER stress response. Amino acids were added at the concentration tested that yielded maximal lifespan extension except phenylalanine, which did not extend lifespan. (A)
Phsp-60::GFP. For panels A and B heat shock at 35°C for 2 hours was used as a positive control. For panels C and D 50 μg/ml ethidium bromide treatment for 2 days was used as a positive control (*p < 0.05 vs. control).