Identification of skin-expressed genes possibly associated with wool growth regulation of Aohan fine wool sheep

BMC Genomic Data

Table 1 Primers used for Q-PCR validation

Gene	Primer sequence (5’-3’)	Tm (°C) ^a	Target size (bp)
GAPDH^b	Forward: ATGCCTCCTGCACCACCA	60	76
GAPDH^b	Reverse: AGTCCCTCCACGATGCCAA	60	76
FGF10	Forward: GATCCGAGAAAGGAGCGAGG	60	554
FGF10	Reverse: TCCAGGATACTGTACGGGCA	60	554
LOC443300	Forward: ACCAACACATCCCATTCGCT	60	140
LOC443300	Reverse: CACTCAGCGTGTCCAGTTCT	60	140
FGF18	Forward: AAGTCCGGATCAAGGGCAAG	60	98
FGF18	Reverse: CACACACTCTTTGCTGGTGC	60	98
Connexin43	Forward: GTCGTGTCGTTGGTGTCTCT	60	291
Connexin43	Reverse: CACTCAGCGTGTCCAGTTCT	60	291
SCD	Forward: AAGAGTGGCTGAGTTTCTGGTC	60	277
SCD	Reverse: GAAAGGAAGGTGATAGGGACAA	60	277
Zo1	Forward: AGATAGCCCTGCAGCCAAAG	60	117
Zo1	Reverse: GGGAGGTCAAGCAGGAAGAG	60	117
MMP2	Forward: AACGCCATCCCTGATAACCT	60	126
MMP2	Reverse: GCTTCCGAACTTCACGCTC	60	126
ITGB1	Forward: AGCACGGATGAGGTGAACAG	60	407
ITGB1	Reverse: CCAAGGCAGGTCTGACACAT	60	407
PAG11	Forward: AGCGTCGCCTACGAATCTG	60	120
PAG11	Reverse: CTCAAACCCATATTCCGTCACA	60	120
CRYAB	Forward: CACCCAGCTGGATTGACACT	60	147
CRYAB	Reverse: CCTCATGTTTGCCATGCACC	60	147

^aThe annealing temperature represents the optimal temperature during quantitative PCR.
^bRNA levels of GAPDH was assayed for normalization during quantitative PCR.

ISSN: 2730-6844