Table 2 The shortest length for a candidate barcode to reach the maximum discrimination success using genetic distance method
Markers | Maximum discrimination success (%) | Shortest length (bp) | Primer name | Primer sequence 5' to 3' |
|---|---|---|---|---|
rbcL | 91.67 | 480 | m-rbcLF | ACAAATTGACTTATTATACTCCTGA |
m-rbcLR | TCGTCTTTGGTAAAATCAAGTCCA | |||
matK | 62.5 | 90 | m-matKF | CTTCTTGAACGAATCTATTTCTA |
m-matKR | CCATAAATTAACAAAGTAATATGT | |||
trnH-psbA | 62.5 | 50 | m-HAF | TAATCTAGAATTTAGCTACTTCTTC |
m-HAR | CCTTGATCCACTTGGCTACATCC | |||
rps16 | 83.33 | 280 | m-rps16F | ATAGGAATGAAGGTGCTCTTG |
m-rps16R | ATCCTTCCAACAAAATGGCAGCA | |||
ycf1a | 91.67 | 60 | m-ycf1aF | TTATTACCGAGTTGGAACAACA |
m-ycf1aR | TTGAGTACGCATAGAACCTTTGAT | |||
ycf1b | 100 | 110 | m-ycf1bF | AAKCAAGAGACAACTTACCTTGA |
m-ycf1bR | GGATCAGATGCACAAAACCAAGGAA |