Tbc1d20 ZFN/ZFNmEF cellular phenotypes. Oleic acid treatment for 24 hr following staining with the neutral lipid dye BODIPY 493/503 revealed expanded LD structures in Tbc1d20 ZFN/ZFN MEFs (B) when compared to control MEFs (A). Quantification analyses shown in (C) identified that % of LD area per cell in Tbc1d20 ZFN/ZFN (13.89 ± 1.23) was significantly greater (P < 0.001) than in control (4.16 ± 0.25) MEFs. P values were determined by Student’s t test and error bars represent SEM. GM130 immunostaining (red) revealed no Golgi differences between Tbc1d20 ZFN/ZFN(E) and control MEFs (D). DNA was stained with DAPI (blue). Scale bars = 5 μm.