Does dietary folic acid supplementation in mouse NTD models affect neural tube development or gamete preference at fertilization?

Table 3 Embryo loss among progeny of NTD mutant intercrosses

Mutant, Diet	Obs. no. embryos			P-value (χ²)	% Lost heterozygous, homozygous	% Lost (combined)	% Resorbed (n)	Ave. litter size** (n)	Reference*
Mutant, Diet	+/+	+/−	−/−	P-value (χ²)	% Lost heterozygous, homozygous	% Lost (combined)	% Resorbed (n)	Ave. litter size** (n)	Reference*
*Lrp6* ^ko
2 ppm	37	73	20	0.04 (6.4)	1.4, 45.9	na	18 (29)	7.9 (20; 4, 12)	1
10 ppm	55	86	16	0.0001 (20.8)	21.8, 70.1	38.2	16 (30)	8.5 (22; 2–12)	1
*Zic2*
2 ppm	56	84	32	0.03 (6.8)	25.0, 42.9	31.0	7.5 (14)	6.1 (28)	2
10 ppm	38	98	36	ns	na	na	11.8 (23)	5.7 (30)	2
*L3P*
2 ppm	28	40	11	0.03 (7.3)	28.6, 60.7	39.3	15.9 (15)	7.2 (13)	2
10 ppm	14	28	16	ns	na	na	18.3 (13)	4.2 (10)	2

See Table 2 for details. ns – not significant, na – not applicable. (Revised from Gray et al. [24] and Marean et al. [25], with permission of the publishers) Bold numbers highlight results of particular interest.
*References: (1) Gray et al. [24], (2) Marean et al. [25]. ** Based on genotyped embryos only.

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