Table 2 Oligonucleotides used for cloning, mutagenesis and EMSA
Denomination of oligonucleotides | Oligonucleotide sequences | PCR product size (bp) | Accession number |
|---|---|---|---|
Oligos used for cloning | |||
pOCTN2-PPRE-HindIII | ATAAAGCTTCAGCCTCTCTGTTTCGTCAG | 228 | CU372899 |
pOCTN2-PPRE-XhoI | ATACTCGAGGAGCTATGTTGCTGCCAGTG | ||
bOCTN2-PPRE-HindIII | ATAAAGCTTCAGAAGGGTCCTTGAGCTAT | 243 | AC149665 |
bOCTN2-PPRE-XhoI | ATACTCGAGCCAACAGTGACTGTTCACCA | ||
hOCTN2-PPRE-HindIII | ATAAAGCTTGCTCTGAACTTCAAGTCAAGC | 246 | AC000137 |
hOCTN2-PPRE-XhoI | ATACTCGAGCTGAGTGATGGTGGCATTGA | ||
Oligos used for mutagenesis and EMSA-mut | |||
pOCTN2-PPREmut-For | AACCTGTAAGTAGGTGTATGGGCACACAACTCGTA | ||
pOCTN2-PPREmut-Rev | TACGAGTTGTGTGCCCATACACCTACTTACAGGTT | ||
bOCTN2-PPREmut-For | AACCTGGAAGTAGGTGTATGGGCACAGAGCTCTTT | ||
bOCTN2-PPREmut-Rev | AAAGAGCTCTGTGCCCATACACCTACTTCCAGGTT | ||
hOCTN2-PPREmut-For | AACATATAAGTAGGTGtAtGGGCACATAACTCCTT | ||
hOCTN2-PPREmut-Rev | AAGGAGTTATGTGCCCaTaCACCTACTTATATGTT | ||
Oligos used for EMSA | |||
pOCTN2-PPRE-For | AACCTGTAAGTAGGTGAAAGGGCACACAACTCGTA | ||
pOCTN2-PPRE-Rev | TACGAGTTGTGTGCCCTTTCACCTACTTACAGGTT | ||
bOCTN2-PPRE-For | AACCTGGAAGTAGGTGAAAGGGCACAGAGCTCTTT | ||
bOCTN2-PPRE-Rev | AAAGAGCTCTGTGCCCTTTCACCTACTTCCAGGTT | ||
hOCTN2-PPRE-For | AACATATAAGTAGGTGAAAGGGCACATAACTCCTT | ||
hOCTN2-PPRE-Rev | AAGGAGTTATGTGCCCTTTCACCTACTTATATGTT | ||
EMSA-sp.-For | TCTTCCCGAACGTGACCTTTGTCCTGGTCCCCTTT | ||
EMSA-sp.-Rev | TCAAAGGGGACCAGGACAAAGGTCACGTTCGGGAA | ||
EMSA-non-sp.-For | TTCCCATCTTGTGAGCTGTCACCCATGGTGGGGTG | ||
EMSA-non-sp.-Rev | CACCCCACCATGGGTGACAGCTCACAAGATGGGAA |