Table 6 Primer sequences and PCR conditions.
Gene | Primer sequences | Step lengths (s) (annealing/extension) | MgCl2 (Mm) | No. of cycles | Ta (°C) |
|---|---|---|---|---|---|
AKR | F: GTCTGCAACTGGTTTATCAAC R: TTAGGCCATGAGTTTTGCCTG | 30/45 | 1.5 | 33 | 50 |
APOA | F: CCTGAAGCTGGCTGACAACC R: TCAGGCCACGGACTTCTGG | 30/45 | 1.5 | 38 | 50 |
BCL-2 | F: GAGAAGAGGCTACGACAACC R: CCATCCTCCGTTGTCCTGG | 30/45 | 1.5 | 33 | 50 |
BCL-X | F: ATGTCCAGCAGTAACCGGG R: CAGCCGCCATTCTCCTGG | 30/45 | 1.5 | 33 | 50 |
BRIP1 | F: GTGGCCAGAAAGTTGATGTAG R: CTATGTGCTAGTACTGTCCAG | 45/60 | 2.5 | 40 | 50 |
CAAX | F: CACTGCTGGAGAGTTCTTCC R: CTAGGAAATCCTGCAGCAC | 45/60 | 1.5 | 35 | 55 |
CXCR4 | F: TCCTCTGGCATACTCATTG R: GCTGGAATGGAAACTTGAAG | 45/60 | 2.5 | 40 | 50 |
EPN2 | F: GACGACTTCATCTATTAGGCG R: AGCCATGGTAGGATGCTGGAG | 45/60 | 2.5 | 40 | 50 |
KELCH | F: CAGACCACTTCATGAACAAAG R: CTTATCATGCAGCGTGGTTG | 45/60 | 2.5 | 40 | 50 |
LEPR | F: CCTGAAACTTTTGAGCAYC R: TTAACAGCTGTTCTCTGTGG | 45/60 | 2.5 | 40 | 50 |
MBL | F: ATCATCAAGCGCATCATCGG R: TCATCTCTCCAGCTCAGGG | 45/60 | 2.5 | 40 | 50 |
MICRO | F: GTGGACGGTGGAGGATTGCTG R: GTGTCAATGCTCTGCGCTTC | 45/60 | 2.5 | 40 | 50 |
NGF | F: ATGCCAGATGGAACAGAAG R: TCAGGGTCTCCCCGATTTC | 30/45 | 1.5 | 38 | 50 |
PKP4 | F: CCTTCAGCAAACGTTGCTAC R: TGTAAGGCTATCTGGACGTG | 45/60 | 2.5 | 40 | 50 |
PPARG | F: GACATGAACTCTTTAAGGATGG R: CTCCACTTAGTATAATGACAGC | 45/60 | 2.5 | 40 | 50 |
TAR | F: TTGCCCAGTCTCTTTGTGGAG R: CTACATTCCCCAGCCTGA | 45/60 | 2.5 | 40 | 50 |
TRANS | F: GAGCTGGTGCAGAAGATCAG R: CTCCGGTATAGAGGAAGGAG | 45/60 | 2.5 | 40 | 50 |
YTH | F: GTGAAGCGCCATGGTCTAC R: GGACTCACCACTTGGAACAG | 45/60 | 2.5 | 40 | 50 |