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Table 1 Oligonucleotide primers used in this study

From: Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha in Japanese Black cattle

Gene

GenBank

Accession

Sequence (5' to 3')

Region

(Exon)

Amplicon

size (bp)

Annealing

temp (°C)

Identified SNPs

FABP4

NC_007312

FW: CTGGACTCAGAGGGTAATAGCATC

RV: TCCAGAGTGAAAAAGAACCATAGGC

1

233

55

 
  

FW: CACACACACACCTGCTCTTTCTTAG

RV: AAAGGTAACATGGTTCAGATCTAGC

2*

397

57

220 bpA/G (I74V)

  

FW: CCATGTTACCTTTGATATTTAGCTG

RV: ACAACGTATCCAGCAGAAAGTCATG

3–4*

1144

59

328 bpG/A (V110M),

348 bpG/C, 396 bpA/G

LXRα

NC_007313

FW: GCACGGTAGCCAAGACATGAGAGTT

RV: GGAGTGAAGAGCCTAAGACAGATGC

2–3

1273

60

45 bpT/C,

152 bpG/A (G51E)

  

FW: CTAGGACAGCGTGGCGTATGAGAGC

RV: ATCTTGGAAAAACAGGTGGGTCTT

4–7

1716

58

397 bpG/A (V133I)

  

FW: CAGTGCAGGAGACCCAGCTTCAATC

RV: TCATCTGGCTCCACCTCTAG

8–10

1060

55

1,314 bpG/T

  

FW: AGGAGTCCAGCACGCCGCAGTCCACT

RV: GGAGTGAAGAGCCTAAGACAGATGC

3*

263

62

 
  

FW: CTAGGACAGCGTGGCGTATGAGAGC

RV: TCCTCTTGCCGCTTCATTTTCTTCA

4*

846

60

 

Cyt b 5

NC_007325

FW: GAATGTCCCAGGACGAGTTCGCA

RV: TCAGTTGAAGTTAAGAGTGGTGGCT

1–4

575

57

195 bpT/C, 201 bpA/G,

360 bpG/A

ACSL4

NC_007331

FW: GTCATAGACATCCCTGGAGCAGACA

RV: CAGACAGTTCAATGCGAGGCTT

2–15

2020

60

 
  

FW: CCTTGGGGAGT TTGTCACAGCAT

RV: AACTGTTAGAGTGAGAGATGCTGTA

2

771

60

 

ACSL1

NC_007328

FW: CCAGGATGATGCAAGCCCACG A

RV: CTCGTTTTGGAAATCATGGATCACC

2–19

2278

56

282 bpC/T, 516 bpC/G,

1,938 bpT/G

  

FW: CCTTGGGGAGTTTGTCACAGCAT

RV: CCTGTCTGGGGAGCACATTTCAG

2

439

58

 

DGAT2

NC_007313

FW: GACCTGTACTGGCTTCGTCATGA

RV: AAACGATTTGCAGATGGTTCCTC

1–8

1154

58

 
  1. * Primers used for genotyping