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Table 5 Amplification details of all 54 SSR loci in different downy mildew species

From: Simple sequence repeat markers useful for sorghum downy mildew (Peronosclerospora sorghi) and related species

Peronosclerospora. Sorghi

P. maydis

P. sacchari

P. philippinesis

Peronospora sparsa

Sclerospora graminicola

DM 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 30, 31, 32, 33, 34, 35, 36, 38, 40, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, & DM 55

DM 1, 5, 6, 7, 8, 9, 11, 12, 13, 14, 16, 19, 20, 21, 23, 24, 26, 27, 28, 30, 31, 32, 33, 34, 35, 36, 37, 38, 40, 41, 43, 44, 45, 46, 47, 49, 51, 52, 53, 54, & DM 55

DM 5, 7, 8, 9, 12, 13, 14, 16, 17, 19, 23, 26, 28, 29, 30, 31, 33, 34, 35, 37, 38, 43, 47, 49, 51, 52, 53, 54, & DM 55

DM 3*, 5, 7, 8*, 9, 10, 11*, 12, 13*, 14, 16, 18*, 19, 22, 26, 27*, 28, 30, 31*, 33, 35, 39*, 40*, 44, 45*, 46, 47, 49, 50*, 52*, 53*, 54*, & DM 55*

DM 2, 3, 7, 9, 12, 13*, 14, 16, 17, 19, 20, 22, 23*, 24*, 25, 27, 30, 31, 35, 36, 40, 41, 43, 45*, 48*, 49, 50*, 51, 53*, & DM 54

DM 2, 3, 4, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19, 20, 22, 23, 25, 27, 29, 30, 31, 35, 37, 38, 39, 40, 42, 45, 46, 48, 49, 51, 52, 53, 54, & DM 55

Total: 50

41

29

33

30

37

  1. Primers in bold letters are cross- amplified in all species
  2. * Primer combinations expressed unique bands differences from other species